Document Detail

Wogonin induces G1 phase arrest through inhibiting Cdk4 and cyclin D1 concomitant with an elevation in p21Cip1 in human cervical carcinoma HeLa cells.
MedLine Citation:
PMID:  19935879     Owner:  NLM     Status:  In-Process    
Wogonin, a naturally occurring flavonoid, has been shown to have tumor therapeutic potential both in vitro and in vivo. To better understand its anticancer mechanism, we examined the effect of wogonin on human cervical carcinoma HeLa cells. In this study, we observed that G1 phase arrest was involved in wogonin-induced growth inhibition in HeLa cells. Over a 24 h exposure of HeLa cells to 90 micromol x L(-1) wogonin, the promoters of G1-S transition, including cyclin D1/Cdk4 and pRb, decreased within 12 h and E2F-1 depleted in the nucleus at the same time. As the G1 phase arrest developed, p53 and the Cdk inhibitor p21Cip1 elevated both at protein and mRNA levels. Furthermore, the up-regulation of p21Cip1 induced by wogonin was dramatically inhibited by siRNA-mediated p53 gene silencing. Collectively, our data suggested that wogonin induced G1 phase arrest in HeLa cells by modulating several key G1 regulatory proteins, such as Cdk4 and cyclin D1, as well as up-regulation of a p53-mediated p21Cip1 expression. This mechanism of wogonin may play an important role in the killing of cancerous cells and offer a potential mechanism for its anticancer action in vivo.
Li Yang; Hai-wei Zhang; Rong Hu; Yong Yang; Qi Qi; Na Lu; Wei Liu; Yi-yi Chu; Qi-dong You; Qing-long Guo
Related Documents :
14652289 - Cell cycle arrest biomarkers in human lung cancer cells after treatment with selenium i...
10662629 - Herpes simplex virus type 1 infection imposes a g(1)/s block in asynchronously growing ...
14975209 - Effect of human osteopontin on proliferation, transmigration and expression of mmp-2 an...
1670579 - Pcna/cyclin expression and brdu uptake define different subpopulations in different cel...
16582629 - In vivo analysis of drosophila deoxyribonucleoside kinase function in cell cycle, cell ...
17224799 - Adenosine deaminase acting on rna 1 accelerates cell cycle through increased translatio...
12079369 - Molecular model for limb development.
8418909 - Immunocytochemical localization of progesterone receptor in the reproductive tract of a...
3542529 - Fluorescence-activated cell analysis of p-fimbriae receptor accessibility on uroepithel...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  87     ISSN:  1208-6002     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  933-42     Citation Subset:  IM    
Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characterization of the O-antigen in the lipopolysaccharide of Cronobacter (Enterobacter) malonaticu...
Next Document:  Antioxidative activities of white rose flower extract and pharmaceutical advantages of its hexane fr...