| Wogonin, an active compound in Scutellaria baicalensis, induces apoptosis and reduces telomerase activity in the HL-60 leukemia cells. | |
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MedLine Citation:
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PMID: 19577445 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Crude extract of Scutellaria baicalensis (S. baicalensis) has cytotoxic effect on human myelogenous leukemia cells (HL-60). We invesigated which compound from the crude extract is responsible for the cytotoxic effect on HL-60 cells. We identified 29 compounds from the crude extract using high performance liquid chromatography mass spectrometry (HPLC/MS). Two of the compounds, baicalin and wogonoside, are converted to baicalein and wogonin, respectively, after treatment with beta-glucuronidase. We observed a dose-dependent reduction in cell viability when cells with either wogonin or aqueous extract of S. baicalensis. Several of the apoptotic features including deoxyribonucleic acid (DNA) fragmentation and increased caspase-3 activity were found in cells treated with wogonin and aqueous extract. The changes were associated with down-regulation of Bcl-2, and not Bax. Furthermore, treatment of HL-60 cells with wogonin or S. baicalensis led to the inhibition of human telomerase reverse transcriptase (hTERT), human telomerase-associated protein 1 (hTP1) and c-myc messenger ribonucleic acid (m-RNA) expression. Wogonin and S. baicaleisis down-regulated the telomerase activity. Our findings suggest that wogonin may be the major compound in S. baicalensis responsible for HL-60 growth inhibition in vitro. The inhibition of HL-60 cell growth is mediated partly through the induction of Bax/Bcl-2 apoptosis and by telomerase inhibition through suppression of c-myc, which is a promoter of hTERT. |
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Authors:
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Sheng-Teng Huang; Chen-Yu Wang; Rong-Chi Yang; Chih-Ju Chu; Hsiao-Ting Wu; Jong-Hwei S Pang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-03 |
Journal Detail:
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Title: Phytomedicine : international journal of phytotherapy and phytopharmacology Volume: 17 ISSN: 1618-095X ISO Abbreviation: Phytomedicine Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9438794 Medline TA: Phytomedicine Country: Germany |
Other Details:
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Languages: eng Pagination: 47-54 Citation Subset: IM |
Affiliation:
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Department of Chinese Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Taiwan. shenteng@adm.cgmh.org.tw |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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