| Wobble inosine tRNA modification is essential to cell cycle progression in G(1)/S and G(2)/M transitions in fission yeast. | |
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MedLine Citation:
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PMID: 17875641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inosine (I) at position 34 (wobble position) of tRNA is formed by the hydrolytic deamination of a genomically encoded adenosine (A). The enzyme catalyzing this reaction, termed tRNA A:34 deaminase, is the heterodimeric Tad2p/ADAT2.Tad3p/ADAT3 complex in eukaryotes. In budding yeast, deletion of each subunit is lethal, indicating that the wobble inosine tRNA modification is essential for viability; however, most of its physiological roles remain unknown. To identify novel cell cycle mutants in fission yeast, we isolated the tad3-1 mutant that is allelic to the tad3(+) gene encoding a homolog of budding yeast Tad3p. Interestingly, the tad3-1 mutant cells principally exhibited cell cycle-specific phenotype, namely temperature-sensitive and irreversible cell cycle arrest both in G(1) and G(2). Further analyses revealed that in the tad3-1 mutant cells, the S257N mutation that occurred in the catalytically inactive Tad3 subunit affected its association with catalytically active Tad2 subunit, leading to an impairment in the A to I conversion at position 34 of tRNA. In tad3-1 mutant cells, the overexpression of the tad3(+) gene completely suppressed the decreased tRNA inosine content. Notably, the overexpression of the tad2(+) gene partially suppressed the temperature-sensitive phenotype and the decreased tRNA inosine content, indicating that the tad3-1 mutant phenotype is because of the insufficient I(34) formation of tRNA. These results suggest that the wobble inosine tRNA modification is essential for cell cycle progression in the G(1)/S and G(2)/M transitions in fission yeast. |
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Authors:
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Satoshi Tsutsumi; Reiko Sugiura; Yan Ma; Hideki Tokuoka; Kazuki Ohta; Rieko Ohte; Akiko Noma; Tsutomu Suzuki; Takayoshi Kuno |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-17 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-12 Completed Date: 2008-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 33459-65 Citation Subset: IM |
Affiliation:
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Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Base Sequence Cell Cycle* Cell Separation Cloning, Molecular Flow Cytometry Inosine / chemistry* Mass Spectrometry Molecular Sequence Data Mutation Phenotype RNA, Transfer / chemistry* Schizosaccharomyces / genetics, metabolism* Sequence Homology, Amino Acid Temperature |
| Chemical | |
Reg. No./Substance:
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58-63-9/Inosine; 9014-25-9/RNA, Transfer |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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