| Withanolide induces apoptosis in HL-60 leukemia cells via mitochondria mediated cytochrome c release and caspase activation. | |
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MedLine Citation:
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PMID: 17328876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study is on the growth inhibitory effect of Withania somnifera methanolic leaf extract and its active component, withanolide on HL-60 promyelocytic leukemia cells. The decrease in survival rate of HL-60 cells was noted to be associated with a time dependent decrease in the Bcl-2/Bax ratio, leading to up regulation of Bax. Both the crude leaf extract and the active component activated the apoptotic cascade through the cytochrome c release from mitochondria. The activation of caspase 9, caspase 8 and caspase 3 revealed that caspase was a key mediator in the apoptotic pathway. DNA fragmentation analysis revealed typical ladders as early as 12h indicative of caspase 3 role in the apoptotic pathway. Flow cytometry data demonstrated an increase of sub-G1 peak upon treatment by 51% at 24h, suggesting the induction of apoptotic cell death in HL-60 cells. |
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Authors:
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V Senthil; S Ramadevi; V Venkatakrishnan; P Giridharan; B S Lakshmi; R A Vishwakarma; A Balakrishnan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-12 |
Journal Detail:
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Title: Chemico-biological interactions Volume: 167 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-20 Completed Date: 2007-05-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: Ireland |
Other Details:
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Languages: eng Pagination: 19-30 Citation Subset: IM |
Affiliation:
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Centre For Biotechnology, Anna University, Chennai 600025, Tamil Nadu, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Caspase 3 / metabolism Cell Proliferation / drug effects Cytochromes c / metabolism DNA Fragmentation Ergosterol / analogs & derivatives*, pharmacology HL-60 Cells Humans Methanol / chemistry Mitochondria / metabolism Plant Extracts / pharmacology Plant Leaves / chemistry Proto-Oncogene Proteins c-bcl-2 / metabolism Rhodamine 123 / metabolism Withania / chemistry* bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/5alpha-ethoxy-1-oxo-6beta,14alpha,17beta,20-tetrahydroxy-20S,22R-witha-2,24-dienolide; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 57-87-4/Ergosterol; 62669-70-9/Rhodamine 123; 67-56-1/Methanol; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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