| Wistar Furth rat megakaryocytes lack dense compartments and intercellular plaques, membranous structures rich in cytoskeletal proteins. | |
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MedLine Citation:
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PMID: 9789686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Wistar Furth (WF) rats have an abnormal thrombopoietic phenotype with morphologically aberrant megakaryocytes, larger than normal mean platelet volume, and platelet alpha-granule protein deficiency. Here, ultrastructural comparisons of WF rat megakaryocytes to those of rats (Wistar) with normal platelet formation during stimulated megakaryocytopoiesis following 5-fluorouracil administration, have revealed a previously unrecognized membrane structure in normal rat megakaryocytes, and two additional abnormalities in WF megakaryocytes. The novel structures were zones of electron density on the cytoplasmic face of apposed plasma membranes of adjoining normal megakaryocytes. These modified focal adhesion-type contacts were distributed at intervals between adjacent megakaryocytes, and were spaced by deposits of extracellular material. These structures also were present between apposed plasma membranes of Wistar rat megakaryocytes in unperturbed marrows, but were absent between megakaryocytes of WF rats. The second WF rat megakaryocyte abnormality is the absence of cytoplasmic dense compartments, another specialized membranous structure that is continuous with the megakaryocyte demarcation membrane system. Both the intercellular plaques and dense compartments of Wistar rat megakaryocytes were found to be rich in cytoskeletal proteins including actin, alpha-actinin, talin, and vinculin as indicated by ultrastructural immunogold labeling. We hypothesize that an abnormality in cytoskeletal protein function may be responsible for the lack of these structures in the WF rat. |
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Authors:
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P E Stenberg; J H Beckstead; C W Jackson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell adhesion and communication Volume: 5 ISSN: 1061-5385 ISO Abbreviation: Cell Adhes. Commun. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-12-22 Completed Date: 1998-12-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9417027 Medline TA: Cell Adhes Commun Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 397-407 Citation Subset: IM |
Affiliation:
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Department of Pathology, Oregon Health Sciences University, Portland, USA. stenberg@ohsu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actinin
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analysis,
immunology Animals Antibodies, Monoclonal Antigens, CD29 / analysis, immunology Antimetabolites / pharmacology Cell Compartmentation / drug effects, physiology* Cytoskeleton / chemistry, physiology* Fluorouracil / pharmacology Megakaryocytes / chemistry, cytology*, ultrastructure Microscopy, Electron Rats Rats, Inbred WF Talin / analysis, immunology Vinculin / analysis, immunology |
| Grant Support | |
ID/Acronym/Agency:
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HL31598/HL/NHLBI NIH HHS; HL51546/HL/NHLBI NIH HHS; P30 CA21765/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD29; 0/Antimetabolites; 0/Talin; 11003-00-2/Actinin; 125361-02-6/Vinculin; 51-21-8/Fluorouracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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