Document Detail


Wireless voltammetry recording in unanesthetised behaving rats.
MedLine Citation:
PMID:  17983679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vivo voltammetry is a valuable technique for rapid measurement of dopamine in the brain of freely behaving rats. Using a conventional voltammetry system, however, behavioural freedom is restricted by cables connecting the head assembly to the measurement system. To overcome these difficulties, we developed a wireless voltammetry system utilizing radio waves. This system consisted of a potentiostat and transmitter system that was mounted on the back of the rat, and a receiver and analysis system. A single-step pulse (100-250 mV) was applied at 4 Hz after an activation pulse to a carbon fibre recording electrode (diameter: 7 microm). Measurement of dopamine (detection limit: 2.7 x 10(-7)M) was demonstrated in vitro. In vivo experiment was performed at least 1 week after the recording electrode was implanted in the rat striatum. Administration of 2-phenylethylamine to rats increased dopamine signal current, which was consistent with the result in the microdialysis measurement. During a resident-intruder test, dopamine signal current in a resident rat increased upon introduction of an intruder rat. These results show that the present wireless system is useful for a long-term measurement of dopamine in behaving rats.
Authors:
Maki Kagohashi; Taizo Nakazato; Kenji Yoshimi; Shunjiro Moizumi; Nobutaka Hattori; Shigeru Kitazawa
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-03
Journal Detail:
Title:  Neuroscience research     Volume:  60     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-07     Completed Date:  2008-04-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  120-7     Citation Subset:  IM    
Affiliation:
Department of Physiology, Juntendo University School of Medicine, Hongo 2-1-1, Tokyo 113-0033, Japan.
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MeSH Terms
Descriptor/Qualifier:
Anesthesia / methods
Animals
Behavior, Animal / drug effects,  physiology
Brain Chemistry / drug effects,  physiology*
Corpus Striatum / drug effects,  metabolism*
Dopamine / analysis*,  metabolism
Electric Stimulation
Electrochemistry / instrumentation*,  methods
Electrodes, Implanted / standards
Electrophysiology / instrumentation,  methods
Extracellular Fluid / chemistry,  metabolism
Male
Microdialysis / instrumentation,  methods
Neurochemistry / instrumentation,  methods*
Phenethylamines / pharmacology
Psychotropic Drugs / pharmacology
Rats
Reaction Time / drug effects,  physiology
Social Behavior
Synaptic Transmission / drug effects,  physiology
Telemetry / instrumentation*
Time Factors
Wakefulness / physiology
Chemical
Reg. No./Substance:
0/Phenethylamines; 0/Psychotropic Drugs; 64-04-0/phenethylamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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