Document Detail


Williopsis saturnus yeast killer toxin does not kill Streptococcus pneumoniae.
MedLine Citation:
PMID:  20967498     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Streptococcus pneumoniae is an important human bacterial pathogen, and the increase in antibiotic resistance demands the development of new antimicrobial compounds. Several reports have suggested that yeast killer toxins show activity against bacteria and we therefore investigated the activity of K9 killer toxin from the yeast Williopsis saturnus var. mrakii NCYC 500 against S. pneumoniae. However, no inhibition of bacterial growth was observed with concentrated K9 preparations in agar diffusion assays and in liquid culture. Although cell morphology was slightly affected by K9 treatment, no effect on cellular viability was detectable, and K9 had no stimulatory effect on cell lysis induced by β-lactams or Triton X-100. This indicated that K9 did not contribute to cell wall damage. Moreover, flow cytometry was used as a sensitive assessment of integrity of cells exposed to killer toxin. No significant damage of S. pneumoniae cells was evident, although minor changes in fluorescence suggested that K9 killer toxin may interact with bacterial surface components.
Authors:
Irma Ochigava; Phillip J Collier; Graeme M Walker; Regine Hakenbeck
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Publication Detail:
Type:  Journal Article     Date:  2010-10-22
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  99     ISSN:  1572-9699     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  559-66     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Kaiserslautern, Paul-Ehrlich St 23, 67663, Kaiserslautern, Germany.
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