Document Detail


Wild type p53 gene causes reorganization of cytoskeleton and, therefore, the impaired deformability and difficult migration of murine erythroleukemia cells.
MedLine Citation:
PMID:  12905527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the role of p53 gene in the biophysics and biology in murine erythroleukemia cell line (MEL), with the goal of understanding the influence of this tumor suppressor gene on the deformability and metastasis of tumor cells. Experiments were performed on MEL and p53-transfected MEL (MEL-M with mutant p53 gene and MEL-W with wild-type p53 gene). The cell growth curves indicated that the over-expression of wild-type p53 gene significantly suppressed the growth of MEL, with G(0)-G(1) arrest and apoptosis shown by flow cytometric assays. Confocal laser scanning microscopy revealed that the MEL-W had a more compact organization of the F-Actin cytoskeleton than MEL and MEL-M. Fluorescence polarization measurement indicated a higher membrane fluidity of MEL-W than the other two groups. Fourier transform infrared spectroscopy (FT-IR) showed changes in the composition and/or structure of membrane lipids in MEL-W, with decreases in secondary structures of proteins such as alpha-helix, turns and bends and random coil, in comparison to MEL and MEL-M. The osmotic fragility curves indicated that MEL-W was more fragile and micropipette experiments showed that they had increased elasticity and reduced deformability in comparison to MEL and MEL-M. The adhesion assay with the use of the flow chamber revealed a lower adhesion rate of MEL-W to endothelial cells at high shear stress. The present study on the molecular biology with biophysics of MEL cells contributes to our knowledge on the tumor suppressor gene p53.
Authors:
Weijuan Yao; Li Gu; Dagong Sun; Weibo Ka; Zongyao Wen; Shu Chien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  56     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-07     Completed Date:  2004-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Affiliation:
School of Basic Medical Sciences, Peking University, Beijing, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Actins / analysis,  metabolism
Animals
Apoptosis / physiology
Blotting, Western
Cell Adhesion / physiology
Cell Cycle / physiology
Cell Division / physiology
Cell Line, Tumor
Cell Movement / physiology*
Cytoskeleton / physiology*
Elasticity
Endothelial Cells / physiology
Flow Cytometry
Fluorescence Polarization
Genes, p53 / genetics,  physiology*
Humans
Leukemia, Erythroblastic, Acute / pathology
Membrane Fluidity / physiology
Mice
Microscopy, Confocal
Mutation
Osmotic Fragility / physiology
Spectroscopy, Fourier Transform Infrared
Transfection
Chemical
Reg. No./Substance:
0/Actins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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