Document Detail


Widespread deregulation of microRNA expression in human prostate cancer.
MedLine Citation:
PMID:  17891175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs (miRNAs) are small regulatory RNAs that can regulate gene expression by binding to mRNA sequences and repressing target-gene expression post-transcriptionally, either by inhibiting translation or promoting RNA degradation. We have analysed expression of 328 known and 152 novel human miRNAs in 10 benign peripheral zone tissues and 16 prostate cancer tissues using microarrays and found widespread, but not universal, downregulation of miRNAs in clinically localized prostate cancer relative to benign peripheral zone tissue. These findings have been verified by real-time RT-PCR assays on select miRNAs, including miR-125b, miR-145 and let-7c. The downregulated miRNAs include several with proven target mRNAs whose proteins have been previously shown to be increased in prostate cancer by immunohistochemistry, including RAS, E2F3, BCL-2 and MCL-1. Using a bioinformatics approach, we have identified additional potential mRNA targets of one of the miRNAs, (miR-125b) that are upregulated in prostate cancer and confirmed increased expression of one of these targets, EIF4EBP1, in prostate cancer tissues. Our findings indicate that changes in miRNA expression may have an important role in the biology of human prostate cancer.
Authors:
M Ozen; C J Creighton; M Ozdemir; M Ittmann
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies     Date:  2007-09-24
Journal Detail:
Title:  Oncogene     Volume:  27     ISSN:  1476-5594     ISO Abbreviation:  Oncogene     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-13     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  1788-93     Citation Subset:  IM    
Affiliation:
Department of Pathology, Baylor College of Medicine, Houston, TX, USA.
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MeSH Terms
Descriptor/Qualifier:
Down-Regulation / genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / physiology*
Humans
Male
MicroRNAs / antagonists & inhibitors*,  biosynthesis*,  metabolism*
Microspheres
Oligonucleotide Array Sequence Analysis
Prostatic Neoplasms / genetics*,  metabolism
Up-Regulation / genetics
Grant Support
ID/Acronym/Agency:
P50CA058204/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/MicroRNAs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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