| Widespread deregulation of microRNA expression in human prostate cancer. | |
| | |
MedLine Citation:
|
PMID: 17891175 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
MicroRNAs (miRNAs) are small regulatory RNAs that can regulate gene expression by binding to mRNA sequences and repressing target-gene expression post-transcriptionally, either by inhibiting translation or promoting RNA degradation. We have analysed expression of 328 known and 152 novel human miRNAs in 10 benign peripheral zone tissues and 16 prostate cancer tissues using microarrays and found widespread, but not universal, downregulation of miRNAs in clinically localized prostate cancer relative to benign peripheral zone tissue. These findings have been verified by real-time RT-PCR assays on select miRNAs, including miR-125b, miR-145 and let-7c. The downregulated miRNAs include several with proven target mRNAs whose proteins have been previously shown to be increased in prostate cancer by immunohistochemistry, including RAS, E2F3, BCL-2 and MCL-1. Using a bioinformatics approach, we have identified additional potential mRNA targets of one of the miRNAs, (miR-125b) that are upregulated in prostate cancer and confirmed increased expression of one of these targets, EIF4EBP1, in prostate cancer tissues. Our findings indicate that changes in miRNA expression may have an important role in the biology of human prostate cancer. |
| | |
Authors:
|
M Ozen; C J Creighton; M Ozdemir; M Ittmann |
Related Documents
:
|
12116365 - Flow cytometric analysis of breast cancer resistance protein expression and function. 12908825 - The expression of dcc protein in female breast cancer. 14689585 - Galectins and urological cancer. 7681615 - Small heat-shock protein is expressed in meningiomas and in granulofilamentous inclusio... 15859165 - Do people with cancer postpone death to celebrate special occasions? 23392085 - Health behaviour advice to cancer patients: the perspective of social network members. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies Date: 2007-09-24 |
Journal Detail:
|
Title: Oncogene Volume: 27 ISSN: 1476-5594 ISO Abbreviation: Oncogene Publication Date: 2008 Mar |
Date Detail:
|
Created Date: 2008-03-13 Completed Date: 2008-04-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
|
Languages: eng Pagination: 1788-93 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Baylor College of Medicine, Houston, TX, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Down-Regulation
/
genetics Gene Expression Profiling Gene Expression Regulation, Neoplastic / physiology* Humans Male MicroRNAs / antagonists & inhibitors*, biosynthesis*, metabolism* Microspheres Oligonucleotide Array Sequence Analysis Prostatic Neoplasms / genetics*, metabolism Up-Regulation / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
P50CA058204/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CD133+ HCC cancer stem cells confer chemoresistance by preferential expression of the Akt/PKB surviv...
Next Document: Interference of the dominant negative helix-loop-helix protein ID1 with the proteasomal subunit S5A ...