Document Detail


Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator.
MedLine Citation:
PMID:  23350531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Time-lapse or longitudinal fluorescence microscopy is broadly used in cell biology. However, current available time-lapse fluorescence microscopy systems are bulky and costly. The limited field-of-view (FOV) associated with the microscope objective necessitates mechanical scanning if a larger FOV is required. Here we demonstrate a wide FOV time-lapse fluorescence self-imaging Petri dish system, termed the Talbot Fluorescence ePetri, which addresses these issues. This system's imaging is accomplished through the use of the Fluorescence Talbot Microscopy (FTM). By incorporating a microfluidic perfusion subsystem onto the platform, we can image cell cultures directly from within an incubator. Our prototype has a resolution limit of 1.2 μm and an FOV of 13 mm(2). As demonstration, we obtained time-lapse images of HeLa cells expressing H2B-eGFP. We also employed the system to analyze the cells' dynamic response to an anticancer drug, camptothecin (CPT). This method can provide a compact and simple solution for automated fluorescence imaging of cell cultures in incubators.
Authors:
Chao Han; Shuo Pang; Danielle V Bower; Patrick Yiu; Changhuei Yang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-02-06
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-19     Completed Date:  2013-11-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2356-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / toxicity
Camptothecin / toxicity
Cell Survival / drug effects
Green Fluorescent Proteins / genetics,  metabolism
HeLa Cells
Histones / genetics,  metabolism
Humans
Microfluidic Analytical Techniques / instrumentation
Microscopy, Fluorescence / instrumentation,  methods*
Temperature
Time-Lapse Imaging*
Grant Support
ID/Acronym/Agency:
1R01AI096226-01/AI/NIAID NIH HHS; F30 HL110723/HL/NHLBI NIH HHS; F30 HL110723/HL/NHLBI NIH HHS; R01 AI096226/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Histones; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; XT3Z54Z28A/Camptothecin
Comments/Corrections

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