Document Detail

Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles.
MedLine Citation:
PMID:  24081309     Owner:  NLM     Status:  MEDLINE    
We present a method for adding molecular specificity to wide-field interferometric phase microscopy (IPM) by recording the phase signatures of gold nanoparticles (AuNPs) labeling targets of interest in biological cells. The AuNPs are excited by time-modulated light at a wavelength corresponding to their absorption spectral peak, evoking a photothermal (PT) effect due to their plasmonic resonance. This effect induces a local temperature rise, resulting in local refractive index and phase changes that can be detected optically. Using a wide-field interferometric phase microscope, we acquired an image sequence of the AuNP sample phase profile without requiring lateral scanning, and analyzed the time-dependent profile of the entire field of view using a Fourier analysis, creating a map of the locations of AuNPs in the sample. The system can image a wide-field PT phase signal from a cluster containing down to 16 isolated AuNPs. AuNPs are then conjugated to epidermal growth factor receptor (EGFR) antibodies and inserted to an EGFR-overexpressing cancer cell culture, which is imaged using IPM and verified by confocal microscopy. To the best of our knowledge, this is the first time wide-field interferometric PT imaging is performed at the subcellular level without the need for total internal reflection effects or scanning.
Nir A Turko; Anna Peled; Natan T Shaked
Related Documents :
23868599 - High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics an...
24136899 - Light sheet based imaging flow cytometry on a microfluidic platform.
24122039 - Formation of targeted monovalent quantum dots by steric exclusion.
22991099 - High-content single-cell analysis on-chip using a laser microarray scanner.
24982819 - Molecular imaging of integrin αvβ6 expression in living subjects.
24396509 - In vivo monitoring of microrna biogenesis using reporter gene imaging.
2688069 - Endoscopic ultrasonography in the assessment of invasive gastric cancer.
18984329 - Comparison of ulnar-shortening osteotomy with a new trimed dynamic compression system v...
24892089 - A method for calculating the area of zostera marina leaves from digital images with noi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-01     Completed Date:  2014-02-04     Revised Date:  2014-07-07    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  111414     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Tumor
Cytological Techniques
Gold / chemistry*
Image Processing, Computer-Assisted / methods*
Interferometry / methods*
Metal Nanoparticles / chemistry*
Microscopy, Phase-Contrast / methods*
Signal Processing, Computer-Assisted
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  miR-34a expression, epigenetic regulation, and function in human placental diseases.
Next Document:  Synthesis and C2-functionalization of indoles with allylic acetates under rhodium catalysis.