| Wide distribution of rat hepatoma asialo GM1 and its different responses to anti-asialo GM1 antibody-mediated in vitro and in situ cell killing. | |
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MedLine Citation:
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PMID: 3112077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By means of TLC immunostaining with anti-asialo GM1 antiserum and conventional structural analyses including exoglycosidase treatment, permethylation and negative ion FABMS, asialo GM1 was found to be widely distributed in rat ascites hepatomas, AH130, AH109A, AH44, AH272, AH41C, AH60C, AH414, AH7974, AH66, AH66 alpha F, AH66F and AH13, and Yoshida sarcoma cells. However, reactivity of asialo GM1, when measured by flow cytometry and complement-mediated cytotoxicity assay with anti-asialo GM1 antiserum, was only observed on AH13 and AH66F, and did not necessarily correspond to the concentration of asialo GM1 on the cells, indicating a cryptic or unreactive nature of glycosphingolipids with respect to their antibodies. On the other hand, although rats injected with 10(7) cells of AH66F died within 7 days, treatment of the rats with anti-asialo GM1 antiserum was found to be effective for their cure or for prolonging their survival, indicating an in situ cytotoxic effect of antiserum. For the in situ cytotoxicity, the timing and period of injection and the dose of antiserum were found to be important. |
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Authors:
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M Iwamori; S Sunada; E Ishihara; H Masago; M Manabe; H Taguchi; H Satoh; Y Nagai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Japanese journal of cancer research : Gann Volume: 78 ISSN: 0910-5050 ISO Abbreviation: Jpn. J. Cancer Res. Publication Date: 1987 Jun |
Date Detail:
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Created Date: 1987-09-24 Completed Date: 1987-09-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8509412 Medline TA: Jpn J Cancer Res Country: JAPAN |
Other Details:
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Languages: eng Pagination: 621-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies* Carbohydrate Sequence Cell Survival / drug effects Flow Cytometry G(M1) Ganglioside* Glycosphingolipids / analysis* Liver Neoplasms, Experimental / analysis* Rats Sarcoma, Yoshida / analysis Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Glycosphingolipids; 37758-47-7/G(M1) Ganglioside; 71012-19-6/asialo GM1 ganglioside |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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