Document Detail

Wide Range of Biotin (Vitamin H) Content in Foodstuffs and Powdered Milks as Assessed by High-performance Affinity Chromatography.
MedLine Citation:
PMID:  24790379     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The biotin (vitamin H) contents of various foodstuffs were determined by using a newly developed high-performance affinity chromatography with a trypsin-treated avidin-bound column. Biotin was derivatized with 9-anthryldiazomethane (ADAM) to fluorescent biotin-ADAM ester. A wide range of biotin contents were found in various foodstuffs depending upon the species (strain), season, organ (of plants and animals), geography, freshness, preparation method and storage method. Among the foodstuffs and fermented foods tested, it was found that wide distributions of biotin content were observed in powdered milk, natto, sake (rice wine), beer, edible oil and sea weed. Since powdered milk is important for child health and development, 14 kinds of powdered and special milks for use in children's diseases were intensively measured. We found that several special milk powders for children with allergies contained low levels of free biotin. Use of these powdered milks caused skin diseases and alopecia in some patients possessing thermolabile serum biotinidase, and administration of free biotin improved their symptoms dramatically. Therefore, it is essential to estimate the total and free biotin contents on each foodstuff in order to improve effective biotin intake and support better health and quality of life for people.
Kou Hayakawa; Noriyuki Katsumata; Kiyomi Abe; Masahiko Hirano; Kazuyuki Yoshikawa; Tsutomu Ogata; Reiko Horikawa; Takeaki Nagamine
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Publication Detail:
Type:  Journal Article     Date:  2009-02-19
Journal Detail:
Title:  Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology     Volume:  18     ISSN:  0918-5739     ISO Abbreviation:  Clin Pediatr Endocrinol     Publication Date:  2009  
Date Detail:
Created Date:  2014-05-05     Completed Date:  2014-05-05     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  9433330     Medline TA:  Clin Pediatr Endocrinol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  41-9     Citation Subset:  -    
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