Document Detail

Wide-angle X-ray scattering as a probe for insulin denaturation.
MedLine Citation:
PMID:  20361993     Owner:  NLM     Status:  MEDLINE    
Wide-angle X-ray scattering (WAXS) from lyophilized protein is characterized by the presence of two relatively broad scattering peaks that are linked to protein structure. This work is concerned with the possibility of utilizing these peaks in the probing of the unfolding and breakdown of insulin. Native insulin is subject to thermal denaturation in the presence and in the absence of thiol catalysts. Denatured products are acid-trapped, lyophilized and monitored using WAXS in addition to Fourier transform infrared spectroscopy (FTIR), gel filtration chromatography and Transmission Electron Microscopy (TEM) as supportive techniques. Results show that the WAXS peak at a d-spacing about 10 A is sensitive towards the alpha-helix content of insulin. A reduction in the intensity of such peak is proven to be directly linked to the reduction of native insulin having normal alpha-helix content. The supportive techniques confirmed the decrease in the alpha-helix content of insulin which accompanied the different denaturation treatments.
Wael M Elshemey; Ibtesam A Mohammad; Anwar A Elsayed
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-31
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  46     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-03     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  471-7     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
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MeSH Terms
Absorption / drug effects
Catalysis / drug effects
Chromatography, Gel
Cysteine / pharmacology
Insulin / chemistry*,  metabolism*
Microscopy, Electron, Transmission
Molecular Probes / chemistry*
Protein Denaturation / drug effects
Scattering, Radiation*
Spectroscopy, Fourier Transform Infrared
Sulfhydryl Compounds / pharmacology
Urea / pharmacology
Reg. No./Substance:
0/Molecular Probes; 0/Sulfhydryl Compounds; 11061-68-0/Insulin; 52-90-4/Cysteine; 57-13-6/Urea

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