Document Detail

Why is inorganic phosphate necessary for uncoupling of oxidative phosphorylation by Cd2+ in rat liver mitochondria?
MedLine Citation:
PMID:  1716986     Owner:  NLM     Status:  MEDLINE    
The phosphate (Pi)-dependent uncoupling action of Cd2+ in oxidative phosphorylation in rat liver mitochondria was studied mainly in terms of Pi transport. Cd2+ at 2 microM caused full uncoupling in the presence of 10 mM Pi, but no uncoupling in the absence of Pi. Cd2+ released state 4 respiration after a certain lag-time, and then the respiration increased progressively with time. After its addition, Cd2+ was taken up by mitochondria in a similar period to the lag time before respiratory release. KIH-201, a potent and specific inhibitor of Pi transport via the Pi/H+ symporter, abolished the uncoupling completely. Cd2+ caused dissipation of the electric transmembrane potential (delta psi) and swelling of mitochondria in a Pi-dependent manner. Uncoupling by Cd2+ was found to take place in parallel with the uptake of Pi into mitochondria via the Pi/H+ symporter, suggesting that the uncoupling was due to acceleration of H+ influx through the Pi/H+ symporter activated by Cd2+.
H Koike; Y Shinohara; H Terada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1060     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-11-18     Completed Date:  1991-11-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-81     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
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MeSH Terms
Biological Transport
Cadmium / metabolism,  pharmacology*
Mitochondria, Liver / drug effects,  metabolism*
Oxidative Phosphorylation*
Phosphates / metabolism*
Rats, Inbred Strains
Ruthenium Red / pharmacology
Reg. No./Substance:
0/Phosphates; 11103-72-3/Ruthenium Red; 7440-43-9/Cadmium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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