| Whole-protein-based enteral formula stimulates intestinal ornithine decarboxylase activity more than single amino acids but does not affect mucosal adenosine triphosphate content in early postsurgical refeeding. | |
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MedLine Citation:
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PMID: 10421389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To restore intestinal integrity after starvation and trauma, luminal nutrients are essential. Specific nutrients such as glutamine support mucosal proliferation and energy metabolism. The aim of this study was to compare effects of enteral formula vs specific amino acids on ornithine decarboxylase (ODC) activity and adenine nucleotide metabolism in jejunal mucosa. METHODS: Male Wistar rats (240 to 280 g) were starved for 48 hours and subjected to intestinal transection, gastrotomy, and jejunal instillation of 5mL nutrient solution. In the first experiment, standard enteral formula (EF) was compared with isonitrogenous formula supplemented with the glutamine precursor, alpha-ketoglutarate (alpha-KG). In a second experiment, 2% glutamine was compared with isonitrogenous ornithine alpha-KG, arginine alpha-KG, glycine and diluted standard enteral formula (EF), or saline. The ODC activity, adenosine triphosphates (ATP), and RNA and protein in the jejunal mucosa were analyzed 2 hours after surgery. RESULTS: The ODC peak in jejunal mucosa in animals treated with EF was higher than when supplemented with alpha-KG (p < .05). Compared with specific amino compounds, EF resulted in a significantly higher ODC peak and no differences were seen between the different specific amino acids. Differences seen in ATP or energy charge between the groups were not significant. CONCLUSIONS: Immediate postoperative enteral feeding by standard EF results in rapid increase of ODC activity. This response was attenuated when the enteral nutrition was supplemented with alpha-KG and was absent when isonitrogenous single amino acids were administered. We found no significant effects on ATP content in the small bowel mucosa by supplementing the diet with alpha-KG. |
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Authors:
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H Yang; M Wirén; J Larsson; J Permert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: JPEN. Journal of parenteral and enteral nutrition Volume: 23 ISSN: 0148-6071 ISO Abbreviation: JPEN J Parenter Enteral Nutr Publication Date: 1999 Jul-Aug |
Date Detail:
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Created Date: 1999-09-09 Completed Date: 1999-09-09 Revised Date: 2007-02-21 |
Medline Journal Info:
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Nlm Unique ID: 7804134 Medline TA: JPEN J Parenter Enteral Nutr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 207-12 Citation Subset: IM |
Affiliation:
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Department of Surgery and Clinical Research Center, Faculty of Health Science, Linköping University, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism* Amino Acids / administration & dosage* Animals Arginine / administration & dosage Energy Metabolism Enteral Nutrition* Food, Formulated* Glutamine / administration & dosage Glycine / administration & dosage Intestinal Mucosa / metabolism Intestines / metabolism* Jejunum / metabolism Ketoglutaric Acids / administration & dosage Male Ornithine / administration & dosage Ornithine Decarboxylase / metabolism* Postoperative Period Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Ketoglutaric Acids; 328-50-7/alpha-ketoglutaric acid; 56-40-6/Glycine; 56-65-5/Adenosine Triphosphate; 56-85-9/Glutamine; 7006-33-9/Ornithine; 74-79-3/Arginine; EC 4.1.1.17/Ornithine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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