Document Detail


Whole genome characterization of reassortant G10P[11] strain (N155) from a neonate with symptomatic rotavirus infection: identification of genes of human and animal rotavirus origin.
MedLine Citation:
PMID:  19505846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Rotavirus G10P[11] strains have long been associated with asymptomatic neonatal infections in some parts of India. We have previously reported G10P[11] strains associated with both asymptomatic infections and severe gastrointestinal disease in neonates from Vellore in southern India, with >90% partial nucleotide and amino acid identity to the VP4, VP6, VP7 and NSP4 genes of the exclusively asymptomatic G10P[11] strain I321.
OBJECTIVES: In this study, the whole genome of a G10P[11] isolate (N155) from a neonate with severe gastrointestinal disease was characterized to determine whether there were significant differences in its genetic makeup in comparison to G10P[11] strain I321 and to establish the origin of the G10P[11] strains in Vellore.
STUDY DESIGN: PCR amplification and complete genome sequencing was carried out for all 11 gene segments of symptomatic G10P[11] rotavirus isolate N155. Nucleotide and amino acid sequence similarity with I321, other human and bovine strains for each gene segment were determined. The origin of each gene was determined based on the degree of identity to bovine or human rotavirus strains.
RESULTS: N155 was found to be a reassortant between human and bovine rotaviruses. With the exception of NSP2, gene sequences of strain N155 showed >90% identity to published sequences of I321. Gene segments encoding NSP1, 2 and 3 were of human rotavirus origin for both strains; however, phylogenetic analysis of NSP2 sequences indicated that the human parental strain that led to the origin of these bovine-human reassortant strains was different. There were no significant differences between NSP2 sequences of strains from symptomatic and asymptomatic neonates in the same setting.
CONCLUSIONS: The study shows that the difference in clinical presentations in neonates may not be due to the limited variability in the genome sequence of G10P[11] strains and that G10P[11] strains in different parts of India could have evolved through reassortment of different parental strains.
Authors:
Sasirekha Ramani; Miren Iturriza-Gomara; Atanu Kumar Jana; Kurien Anil Kuruvilla; James J Gray; David W Brown; Gagandeep Kang
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Publication Detail:
Type:  Case Reports; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-07
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  45     ISSN:  1873-5967     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-30     Completed Date:  2009-08-11     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Affiliation:
Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU200793;  EU200794;  EU200795;  EU200796;  EU200797;  EU200798;  EU200799;  EU200800;  EU200801;  EU200802;  EU200803
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Gastroenteritis / virology
Genome, Viral*
Humans
India
Infant, Newborn
Male
Molecular Sequence Data
Phylogeny
RNA, Viral / genetics*
Reassortant Viruses / genetics*
Rotavirus / genetics*,  isolation & purification*
Rotavirus Infections / virology*
Sequence Analysis, DNA
Sequence Homology
Viral Proteins / genetics
Grant Support
ID/Acronym/Agency:
063144//Wellcome Trust
Chemical
Reg. No./Substance:
0/RNA, Viral; 0/Viral Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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