Document Detail

Whole-cell biocatalysis for 1-naphthol production in liquid-liquid biphasic systems.
MedLine Citation:
PMID:  19700554     Owner:  NLM     Status:  MEDLINE    
Whole-cell biocatalysis to oxidize naphthalene to 1-naphthol in liquid-liquid biphasic systems was performed. Escherichia coli expressing TOM-Green, a variant of toluene ortho-monooxygenase (TOM), was used for this oxidation. Three different solvents, dodecane, dioctyl phthalate, and lauryl acetate, were screened for biotransformations in biphasic media. Of the solvents tested, lauryl acetate gave the best results, producing 0.72 +/- 0.03 g/liter 1-naphthol with a productivity of 0.46 +/- 0.02 g/g (dry weight) cells after 48 h. The effects of the organic phase ratio and the naphthalene concentration in the organic phase were investigated. The highest 1-naphthol concentration (1.43 g/liter) and the highest 1-naphthol productivity (0.55 g/g [dry weight] cells) were achieved by optimization of the organic phase. The ability to recycle both free cells and cells immobilized in calcium alginate was tested. Both free and immobilized cells lost more than approximately 60% of their activity after the first run, which could be attributed to product toxicity. On a constant-volume basis, an eightfold improvement in 1-naphthol production was achieved using biphasic media compared to biotransformation in aqueous media.
S V B Janardhan Garikipati; Angela M McIver; Tonya L Peeples
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-08-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  75     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-08     Completed Date:  2009-12-01     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6545-52     Citation Subset:  IM    
Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 52242, USA.
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MeSH Terms
Cells, Immobilized
Chemical Engineering
Escherichia coli / drug effects,  genetics,  metabolism
Mixed Function Oxygenases / genetics,  metabolism
Naphthalenes / metabolism,  toxicity
Naphthols / metabolism*,  toxicity
Recombinant Proteins / genetics,  metabolism
Reg. No./Substance:
0/Acetates; 0/Naphthalenes; 0/Naphthols; 0/Recombinant Proteins; 0/Solvents; 112-66-3/lauryl acetate; 90-15-3/1-naphthol; 91-20-3/naphthalene; EC 1.-/Mixed Function Oxygenases; EC 1.13.12.-/toluene ortho-monooxygenase

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