Document Detail

Whole-cell NMR characterization of two photochemically active states of the photosynthetic reaction center in heliobacteria.
MedLine Citation:
PMID:  22746282     Owner:  NLM     Status:  Publisher    
Applying photo-CIDNP (photochemically induced dynamic nuclear polarization) MAS (magic-angle spinning) NMR to whole cells of Heliobacillus mobilis, we demonstrate that heliobacterial reaction centers (RCs) are operational in two different states as indicated by the occurrence of a light-induced spin-correlated radical pair. A culture maintained anaerobically is called "Braunstoff" (German for "brown substance"). After exposure to oxygen, Braunstoff is converted to "Grünstoff" ("green substance") as indicated by a color change due to the conversion of BChl g to Chl aF. It is shown that electron transfer occurs symmetrically via both branches of cofactors in both forms. The donor and acceptor cofactors remain identical and unchanged upon conversion while the intermediate accessory cofactors are transformed from BChl g to Chl aF. The donor triplet state in Braunstoff is localized on the special pair donor and lives for 100 μs, demonstrating the absence of nearby carotenoids. In Grünstoff, the donor triplet becomes mobile and appears to be formed on an accessory cofactor.
Smitha Surendran Thamarath; Alia Alia; Eugenio Daviso; Deni Mance; John Harvey Golbeck; Jörg Matysik
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-2
Journal Detail:
Title:  Biochemistry     Volume:  -     ISSN:  1520-4995     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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