Document Detail


Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats.
MedLine Citation:
PMID:  19074373     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from alpha-linolenic acid (alpha-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rates in humans and nonhumans are unknown. We estimated whole-body (presumably liver) synthesis rates in unanesthetized rats by infusing [U-(13)C]alpha-LNA intravenously for 2 h and measuring labeled and unlabeled n-3 PUFA in arterial plasma using negative chemical ionization GC-MS. Newly synthesized esterified [(13)C]DHA, [(13)C]EPA, and [(13)C]docosapentaenoic acid (DPA) appeared in arterial plasma after 60 min of infusion, then their concentrations rose in an S-shaped manner. Esterified concentration x plasma volume data were fit with a sigmoidal equation, whose peak first derivatives provided synthesis rates of unlabeled EPA, DPA, and DHA equal to 8.40, 6.27, and 9.84 mumol/day, respectively. The DHA synthesis rate exceeded the published daily rat brain DHA consumption rate by 30-fold, suggesting that liver synthesis from alpha-LNA could maintain brain DHA homeostasis were DHA absent from the diet. This stable isotope infusion method could be used to quantify whole-body DHA synthesis rates in human subjects.
Authors:
Fei Gao; Dale Kiesewetter; Lisa Chang; Kaizong Ma; Jane M Bell; Stanley I Rapoport; Miki Igarashi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Retracted Publication     Date:  2008-12-11
Journal Detail:
Title:  Journal of lipid research     Volume:  50     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-19     Completed Date:  2009-06-22     Revised Date:  2014-07-22    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  749-58     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism
Carbon Isotopes
Docosahexaenoic Acids / blood,  metabolism
Eicosapentaenoic Acid / biosynthesis,  blood
Fatty Acids, Omega-3 / biosynthesis*,  blood
Homeostasis
Humans
Kinetics
Liver / metabolism
Male
Models, Biological
Rats
Rats, Inbred F344
Species Specificity
alpha-Linolenic Acid / blood*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids, Omega-3; 0RBV727H71/alpha-Linolenic Acid; 25167-62-8/Docosahexaenoic Acids; AAN7QOV9EA/Eicosapentaenoic Acid
Comments/Corrections
Retraction In:
J Lipid Res. 2014 May;55(5):991   [PMID:  24771869 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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