Document Detail

Whole blood interleukin 8 and plasma interleukin 8 levels in newborn infants with suspected bacterial infection.
MedLine Citation:
PMID:  15174789     Owner:  NLM     Status:  MEDLINE    
AIM: To evaluate a new micro-method technique for measurement of interleukin 8 in detergent-lysed whole blood (whole blood IL-8) applicable to capillary blood sampling as a test for bacterial infections in neonates. METHODS: Whole blood IL-8 was measured at the first suspicion of infection along with IL-8 determined in plasma (plasma IL-8), C-reactive protein and blood cultures in 154 consecutive newborn infants with clinical signs of bacterial infection. Only 20 microl of whole blood were required for the new assay. RESULTS: Blood culture-proven infections were diagnosed in six infants and clinical infection (defined as a combination of clinical and laboratory signs) in 20 infants. 1000 pg/ml was determined as the suitable threshold for whole blood IL-8 by ROC-curve analysis. At that threshold, whole blood IL-8 detected blood culture-proven infections with a sensitivity of 83% and a specificity of 67%. The areas under the ROC curves were similar for whole blood IL-8 and plasma IL-8. CONCLUSIONS: Compared with plasma IL-8, whole blood IL-8 offers the advantages that measurements of whole blood IL-8 require a smaller blood sample volume and are not altered by haemolysis. The diagnostic accuracy of whole blood IL-8 remains to be studied in more detail in the future.
A R Franz; S Sieber; F Pohlandt; M Kron; G Steinbach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta paediatrica (Oslo, Norway : 1992)     Volume:  93     ISSN:  0803-5253     ISO Abbreviation:  Acta Paediatr.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-03     Completed Date:  2004-10-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9205968     Medline TA:  Acta Paediatr     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  648-53     Citation Subset:  IM    
Department of Paediatrics, Division of Neonatology and Paediatric Critical Care, University of Ulm, 89070 Ulm, Germany.
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MeSH Terms
Bacterial Infections / blood,  diagnosis*
Biological Markers
C-Reactive Protein / analysis
Infant, Newborn
Interleukin-8 / blood*,  diagnostic use
Prospective Studies
Sensitivity and Specificity
Reg. No./Substance:
0/Biological Markers; 0/Interleukin-8; 9007-41-4/C-Reactive Protein

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