Document Detail


Whole blood folate concentrations: comparison between Stratus Folate (DADE) and radioassay (DPC) methods.
MedLine Citation:
PMID:  9368797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The analytical performance of the Stratus Folate assay for intra-erythrocyte folate determination in normal subjects and in patients affected by folate-related diseases was compared with that of the radioassay (DPC) routinely employed by us. Folate concentrations were measured in freshly obtained EDTA whole blood from 100 subjects. Haemolysis was performed with the appropriate lysis reagent. In addition, to compare two different haemolysis procedures folate determination was carried out in 51 samples haemolysed according to the two procedures in parallel. Data were analyzed using Wilcoxon's test and standardized principal component analysis. Stratus Folate assay and radioassay performances were comparable in terms of analytical characteristics as well as in individual intraerythrocyte folate values across the range of whole blood concentrations examined in the survey. Significant differences were detected between the two different haemolysis procedures only for the radioassay. In conclusion, we observed no significant differences between the two folate determination methods despite their different analytical principles, which indicates the suitability of routine use of the automated non-isotopic Stratus Folate assay for clinical purposes. Moreover, with the latter assay the laboratory staff could choose the more convenient haemolysis procedure.
Authors:
F Bamonti-Catena; A Porcella; M Pomati; C Franzini; M Rosina; V Cavalca; A T Maiolo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies     Volume:  35     ISSN:  0939-4974     ISO Abbreviation:  Eur J Clin Chem Clin Biochem     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-01-21     Completed Date:  1998-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105775     Medline TA:  Eur J Clin Chem Clin Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  781-5     Citation Subset:  IM    
Affiliation:
Istituto di Scienze Mediche, Università di Milano, Ospedale Maggiore-IRCCS, Italy. Filobus@IMIUCCA.CSI.UNIMI.IT
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MeSH Terms
Descriptor/Qualifier:
Automation
Edetic Acid
Erythrocytes / metabolism*
Fasting / blood
Fluorometry / methods
Folic Acid / blood*
Hemolysis
Humans
Radioimmunoassay / methods
Chemical
Reg. No./Substance:
59-30-3/Folic Acid; 60-00-4/Edetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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