Document Detail


Whole genome expression profiling reveals a significant role for the cell junction and apoptosis pathways in breast cancer stem cells.
MedLine Citation:
PMID:  20082156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Side population (SP) cells in primary tumors and cell lines are a small cell population, but they are known to enrich cancer stem cells (CSCs). In this study, we isolated SP cells from the human breast cancer cell line MCF7 as a model for studying CSCs. Compared with non-SP cells, MCF7 SP cells had higher mammosphere-formation efficiency (MFE) in vitro and greater tumorigenicity in vivo, suggesting that MCF7 SP cells enrich CSCs. We first directly compared the gene expression profile of SP and non-SP cells from MCF7 cell line. Comparing the expression signature of SP to non-SP cells, we identified 753 differentially expressed genes (DEGs). Using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, we identified multiple pathways that were aberrantly regulated in SP compared with non-SP cells. Several pathways, including cell junction and apoptosis, play important roles in breast CSC function. This study demonstrates that combining global gene expression analysis with detailed annotated pathway resources can enhance our understanding of the critical pathways that regulate breast CSCs.
Authors:
Mingzhu Huang; Yuqing Li; Guohua Wu; Fengchun Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biotechnology     Volume:  45     ISSN:  1559-0305     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-31     Completed Date:  2010-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-48     Citation Subset:  IM    
Affiliation:
Department of Radiotherapy, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. mingzhuhuang0718@163.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics*
Breast Neoplasms / genetics*
Cell Line, Tumor
Chromosome Mapping / methods
Gene Expression Profiling / methods
Genome / genetics*
Intercellular Junctions / genetics*
Mice
Mice, SCID
Signal Transduction / genetics*
Stem Cells*
Tumor Markers, Biological / genetics*
Chemical
Reg. No./Substance:
0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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