| White matter damage and chemokine induction in developing rat brain after intrauterine infection. | |
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MedLine Citation:
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PMID: 16238536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to investigate the neuropathological effects on the developing rat brain after intrauterine infection, identification of glail fibrillary acidic protein (GFAP), 2', 3'-cyclic nucleotide phosphodiesterase (CNPase), and neurofilament (NF) was observed. Escherichia coli (E. coli) was inoculated into uterine horn of pregnant rats when gestation was 70% complete (15 days) and the control group was inoculated with normal saline. Immunohistochemistry was used for evaluation of GFAP, CNPase, and NF expression in pup brains at postnatal day 7 (P7) and reverse transcriptase-PCR (RT-PCR) to analyze macrophage inflammatory protein-1 alpha mRNA (MIP-1 alpha mRNA), macrophage inflammatory protein-1 beta mRNA (MIP-1beta mRNA), the regulated upon activation normal T expressed and secreted chemokine mRNA (RANTES mRNA) and Eotaxin mRNA expression in pup brains at P1, P3 and P7. The numbers of GFAP-positive cells of the E. coli-treated group pups were marked increased in periventricular white matter and hippocampus at P7 compared with the control group but no significant different levels of GFAP expression in corpus callosum were found between two groups. The integrate density (ID) of CNPase-positive staining of the Escherichia coli-treated group pups were marked decreased in periventricular white matter and corpus callosum at P7 compared with the control group. The ID of NF-positive staining of the Escherichia coli-treated group pups were marked decreased in periventricular white matter at P7 compared with the control group and no significant different levels of NF expression in corpus callosum were found between two groups. The expression of MIP-1 alpha mRNA and MIP-1 beta mRNA in brain of the E. coli-treated pup rat were higher than the control at P1, but the expression of MIP-1 alpha mRNA and MIP-1 beta mRNA in brain of the pup rat at P3 and P7 had no significant difference between two groups. The alteration of expression of GFAP, CNPase, and NF in the brain of neonatal rats after intrauterine infection suggested that intrauterine infection could cause neonatal white matter damage. Moreover, the transient increase in expression of chemokine such as MIP-1 alpha, MIP-1 beta in neonatal brain after intrauterine infection indicated that MIP-1 alpha, MIP-1 beta may be a mechanism mediating between the neonatal white matter damage and the intrauterine infection. |
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Authors:
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Tian-Ming Yuan; Hui-Min Yu; Wei-Zhong Gu; Jian-Ping Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of perinatal medicine Volume: 33 ISSN: 0300-5577 ISO Abbreviation: J Perinat Med Publication Date: 2005 |
Date Detail:
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Created Date: 2005-10-21 Completed Date: 2005-12-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0361031 Medline TA: J Perinat Med Country: Germany |
Other Details:
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Languages: eng Pagination: 415-22 Citation Subset: IM |
Affiliation:
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Department of Neonatology, Laboratory, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2',3'-Cyclic-Nucleotide Phosphodiesterases
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metabolism Animals Animals, Newborn Brain / metabolism* Chemokine CCL4 Chemokines / metabolism* Escherichia coli Infections / metabolism*, microbiology Female Glial Fibrillary Acidic Protein / metabolism Immunochemistry Macrophage Inflammatory Proteins / metabolism Pregnancy Pregnancy Complications, Infectious / metabolism*, microbiology RNA, Messenger / analysis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL4; 0/Chemokines; 0/Glial Fibrillary Acidic Protein; 0/Macrophage Inflammatory Proteins; 0/RNA, Messenger; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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