Document Detail


White gels: An easy way to preserve methylene blue stained gels.
MedLine Citation:
PMID:  22107889     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Methylene blue can be used as a stain for visualizing nucleic acids in polyacrylamide gel electrophoresis. However, its relatively low sensitivity and reversible binding make it a temporary stain that diffuses from the gel relatively fast. Here we describe a very simple method for fixing methylene blue bands in nucleic acid polyacrylamide gels. The procedure makes the methylene blue stain permanent and increases the visibility of the bands, also contributing to increasing the sensitivity of methylene blue.
Authors:
Ana Maria Soto; David Draper
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-4
Journal Detail:
Title:  Analytical biochemistry     Volume:  -     ISSN:  1096-0309     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Chemistry, Towson University, Towson, MD 21252, USA.
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