| Which extractant should be used for the screening and isolation of antimicrobial components from plants? | |
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MedLine Citation:
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PMID: 9533426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Freeze dried and finely ground leaves of two plants with known antimicrobial activity, Anthocleista grandiflora and Combretum erythrophyllum were extracted with acetone, ethanol, methanol, methylenedichloride, methanol/chloroform/water and water at a 1 to 10 ratio in each case. The quantity and diversity of compounds extracted, number of inhibitors extracted, rate of extraction, toxicity in a bioassay, ease of removal of solvent and biological hazard were evaluated for each extractant. An arbitrary scoring system was developed to evaluate the above parameters for the different extractants. Acetone gave the best results with these plants with an arbitrary value of 102 followed by methanol/chloroform/water (81), methylene dichloride (79), methanol (71), ethanol (58) and water (47). Four five minute sequential extractions of very finely ground A. grandiflora shaking at a high rate extracted 97% of the total antimicrobial activity. |
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Authors:
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J N Eloff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: 60 ISSN: 0378-8741 ISO Abbreviation: J Ethnopharmacol Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-05-14 Completed Date: 1998-05-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: IRELAND |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Pretoria, South Africa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Infective Agents
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isolation & purification,
pharmacology* Ethanol Methanol Microbial Sensitivity Tests Plant Extracts* Plants, Medicinal / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Plant Extracts; 64-17-5/Ethanol; 67-56-1/Methanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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