| Whey and casein labeled with L-[1-13C]leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion. | |
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MedLine Citation:
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PMID: 21045172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Muscle protein turnover following resistance exercise and amino acid availability are relatively well described. By contrast, the beneficial effects of different sources of intact proteins in relation to exercise need further investigation. Our objective was to compare muscle anabolic responses to a single bolus intake of whey or casein after performance of heavy resistance exercise. Young male individuals were randomly assigned to participate in two protein trials (n = 9) or one control trial (n = 8). Infusion of l-[1-(13)C]leucine was carried out, and either whey, casein (0.3 g/kg lean body mass), or a noncaloric control drink was ingested immediately after exercise. l-[1-(13)C]leucine-labeled whey and casein were used while muscle protein synthesis (MPS) was assessed. Blood and muscle tissue samples were collected to measure systemic hormone and amino acid concentrations, tracer enrichments, and myofibrillar protein synthesis. Western blots were used to investigate the Akt signaling pathway. Plasma insulin and branched-chain amino acid concentrations increased to a greater extent after ingestion of whey compared with casein. Myofibrillar protein synthesis was equally increased 1-6 h postexercise after whey and casein intake, both of which were higher compared with control (P < 0.05). Phosphorylation of Akt and p70(S6K) was increased after exercise and protein intake (P < 0.05), but no differences were observed between the types of protein except for total 4E-BP1, which was higher after whey intake than after casein intake (P < 0.05). In conclusion, whey and casein intake immediately after resistance exercise results in an overall equal MPS response despite temporal differences in insulin and amino acid concentrations and 4E-BP1. |
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Authors:
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Søren Reitelseder; Jakob Agergaard; Simon Doessing; Ida C Helmark; Peter Lund; Niels B Kristensen; Jan Frystyk; Allan Flyvbjerg; Peter Schjerling; Gerrit van Hall; Michael Kjaer; Lars Holm |
Publication Detail:
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Type: Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't Date: 2010-11-02 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 300 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-29 Completed Date: 2011-01-31 Revised Date: 2011-04-05 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E231-42 Citation Subset: IM |
Affiliation:
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Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, Copenhagen NV, Denmark. s.reitelseder@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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metabolism Adult Amino Acids / blood, metabolism Carbon Isotopes Caseins / chemistry, metabolism* Dietary Proteins / metabolism* Food, Formulated / analysis* Humans Insulin / blood Leucine / blood, chemistry, metabolism* Male Milk Proteins / chemistry, metabolism* Muscle Proteins / biosynthesis* Myofibrils / metabolism Phosphoproteins / metabolism Phosphorylation Proto-Oncogene Proteins c-akt / metabolism Resistance Training* Ribosomal Protein S6 Kinases, 70-kDa / metabolism Signal Transduction Single-Blind Method |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Amino Acids; 0/Carbon Isotopes; 0/Caseins; 0/Dietary Proteins; 0/EIF4EBP1 protein, human; 0/Milk Proteins; 0/Muscle Proteins; 0/Phosphoproteins; 0/whey protein; 11061-68-0/Insulin; 61-90-5/Leucine; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa |
| Comments/Corrections | |
Comment In:
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Am J Physiol Endocrinol Metab. 2011 Mar;300(3):E610; author reply E611-2
[PMID:
21357461
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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