Document Detail


Wheat sprout extract-induced apoptosis in human cancer cells by proteasomes modulation.
MedLine Citation:
PMID:  19527768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Natural occurring modulators of proteasome functionality are extensively investigated for their implication in cancer therapy. On the basis of our previous evidences both on proteasomal inhibition by monomeric polyphenols, and on the characterization of wheat sprout hydroalcoholic extract, herein we thoroughly report on a comparative study of the effect of wheat sprout extract on both normal and tumour cells. Treatment of isolated 20S proteasomes with wheat sprout extracts induced a gradual inhibition of all proteasome activities. Next, two wheat sprout extract components were separated: a polyphenol and a protein fraction. Both components exerted an in vitro inhibitory effect on proteasome activity. HeLa tumour cells and FHs 74 Int normal cells were exposed to both fractions, resulting in different rates of proteasome inhibition, with tumour cells showing a significantly higher degree of proteasome impairment and apoptosis induction. Furthermore, a decrease in proteasome activities and in cell survival of the human plasmacytoma RPMI 8226 cell line, upon the same treatments, was observed. Collectively, our results provide additional evidences supporting the possible use of natural extracts as coadjuvants in cancer treatments.
Authors:
Laura Bonfili; Manila Amici; Valentina Cecarini; Massimiliano Cuccioloni; Rosalia Tacconi; Mauro Angeletti; Evandro Fioretti; Jeffrey N Keller; Anna Maria Eleuteri
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Publication Detail:
Type:  Journal Article     Date:  2009-06-13
Journal Detail:
Title:  Biochimie     Volume:  91     ISSN:  1638-6183     ISO Abbreviation:  Biochimie     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  1131-44     Citation Subset:  IM    
Affiliation:
Department of Molecular, Cellular and Animal Biology, University of Camerino, 62032 Camerino, MC, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Blotting, Western
Cattle
Cell Line, Tumor
Cell Survival / drug effects
Hela Cells
Humans
Plant Extracts / isolation & purification,  pharmacology*
Poly(ADP-ribose) Polymerases / metabolism
Proteasome Endopeptidase Complex / drug effects*,  metabolism
Triticum / chemistry*
Chemical
Reg. No./Substance:
0/Plant Extracts; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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