| What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues. | |
| | |
MedLine Citation:
|
PMID: 19016008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell membranes can be transiently permeabilized under application of electric pulses. This treatment allows hydrophilic therapeutic molecules, such as anticancer drugs and DNA, to enter into cells and tissues. This process, called electropermeabilization or electroporation, has been rapidly developed over the last decade to deliver genes to tissues and organs, but there is a general agreement that very little is known about what is really occurring during membrane electropermeabilization. It is well accepted that the entry of small molecules, such as anticancer drugs, occurs mostly through simple diffusion after the pulse while the entry of macromolecules, such as DNA, occurs through a multistep mechanism involving the electrophoretically driven interaction of the DNA molecule with the destabilized membrane during the pulse and then its passage across the membrane. Therefore, successful DNA electrotransfer into cells depends not only on cell permeabilization but also on the way plasmid DNA interacts with the plasma membrane and, once into the cytoplasm, migrates towards the nucleus. The focus of this review is to describe the different aspects of what is known of the mechanism of membrane permeabilization and associated gene transfer and, by doing so, what are the actual limits of the DNA delivery into cells. |
| | |
Authors:
|
Jean-Michel Escoffre; Thomas Portet; Luc Wasungu; Justin Teissié; David Dean; Marie-Pierre Rols |
Related Documents
:
|
7518788 - Structure and organization of a stable extrachromosomal element in human cells. 20633618 - Phosphorylatable short peptide conjugation for facilitating transfection efficacy of cs... 22502888 - Relationship between plasmid size and shock wave-mediated bacterial transformation. 16691028 - Dna-spermine and dna-lipid aggregate formation visualized by fluorescence correlation s... 321428 - Conservation of transfer ribonucleic acid and 5s ribonucleic acid cistrons in enterobac... 196648 - Studies on viral dna protein complexes isolated at different times after infection of m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2008-11-18 |
Journal Detail:
|
Title: Molecular biotechnology Volume: 41 ISSN: 1073-6085 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-01-21 Completed Date: 2009-08-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 286-95 Citation Subset: IM |
Affiliation:
|
CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205, Route de Narbonne, 31077 Toulouse, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Membrane Permeability* Electrochemotherapy Electroporation* Gene Expression Gene Transfer Techniques* Humans Mice Plasmids / chemistry |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The NADP-dependent glutamate dehydrogenase gene from the astaxanthin producer Xanthophyllomyces dend...
Next Document: Immunohistochemical expression of PTEN and phosphorylated Akt are not correlated with clinical outco...