| What is the optimal anesthetic protocol for measurements of cerebral autoregulation in spontaneously breathing mice? | |
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MedLine Citation:
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PMID: 20972776 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Autoregulation, an important feature of the cerebral circulation, is affected in many diseases. Since genetically modified mice are a fundamental tool in biomedical research, including neuro(bio)logy also in this specie measurements of cerebral autoregulation (CA) are mandatory. However, this requires anesthesia that unfortunately significantly impacts cerebral perfusion and consequently might distort CA measurements directly or by altering arterial pCO(2). The latter can be avoided by artificial ventilation but requires several control measurements of blood gases, each consuming at least 100 μl of blood or 5% of a mouse's blood volume. To avoid such diagnostic hemorrhage, we systematically analyzed the effect of different common anesthetic protocols used for rodents in spontaneously breathing mice on CA measured with Laser speckle perfusion imaging. Halothane, Isoflurane and Pentobarbital abrogated CA and Ketamin/Xylazine as well as Chloralose had a moderate reproducibility. In contrast, the rather rarely used anesthetic Ethomidate applied in low doses combined with local anesthetics had the best reproducibility. Although with this anesthesia the lower CA limit was lower than with Ketamin/Xylazine and Chloralose as reported in the handful of papers so far dealing with CA in mice, we suggest Ethomidate as the anesthetic of choice for CA measurements in spontaneously breathing mice. |
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Authors:
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Zhenghui Wang; Beat Schuler; Olga Vogel; Margarete Arras; Johannes Vogel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-24 |
Journal Detail:
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Title: Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale Volume: 207 ISSN: 1432-1106 ISO Abbreviation: Exp Brain Res Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0043312 Medline TA: Exp Brain Res Country: Germany |
Other Details:
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Languages: eng Pagination: 249-58 Citation Subset: IM |
Affiliation:
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Institute of Veterinary Physiology, Vetsuisse Faculty University of Zürich and Zürich Center for Integrative Human Physiology (ZIHP), Winterthurerstr. 260, 8057, Zürich, Switzerland. |
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