| What happens to tears inside the efferent lacrimal passage? An animal experimental study. | |
| | |
MedLine Citation:
|
PMID: 10943674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: To determine whether absorption of protein components of the tear fluid occurs in normal efferent tear ducts, an animal experiment was carried out. METHODS: Iodinated albumin was dropped into eyes of female rats. After 10, 20 or 60 min the rats were killed, blood collected and the heads embedded for histological examination. Serum was obtained from the clotted blood and the radioactivity in a protein sediment and the combined supernatants counted. In a second approach, serum was fractionated by molecular mass and radioactivity in the fractions measured. Furthermore, autoradiographs of rat head sections were performed. RESULTS: Uptake of radioactivity into the serum was low, but increased with time. After 60 min maximal incorporation of the applied radioactivity into the blood was 0.13%; most (70-80%) of the incorporated radioactivity was not protein bound. Gel chromatographic separation according to molecular mass yielded fractionated peaks of radioactivity corresponding to albumin with maximal 4.8 Bq/ml serum, iodinated tyrosine (5.5 Bq/ml), and free iodine (237 Bq/ml; each after 60 min). Histologically the rat efferent lacrimal tear ducts showed a multilayered lining epithelium with integrated goblet cells in a characteristic arrangement of several cells. In autoradiographs of rat head sections no transport of radioactivity could be visualized. CONCLUSION: In rats only traces of iodinated albumin are incorporated from the efferent lacrimal tear ducts into the blood. A higher proportion of the radioactivity is taken up as the proteolytic degradation product of bovine serum albumin to free amino acids, and 96% of the radioactivity incorporated was free iodine, probably as a contaminant of the iodinated preparation. |
| | |
Authors:
|
F Paulsen; A Thale; R Mentlein |
Related Documents
:
|
2339984 - Tissue-specific regulation of the expression of rat kallikrein gene family members by t... 19362454 - Pubertal exposure to saisentong: effects on thyroid and hepatic enzyme activity in juve... 11829324 - Body, heart, thyroid gland and skeletal muscle weight changes in rats with altered thyr... 17510084 - Enhanced thyroid carcinogenicity of n-nitrosobis(2-oxopropyl)amine in otsuka long-evans... 2506104 - Influence of infusate viscosity on intestinal absorption in the rat. an explanation of ... 1907734 - Effect of dietary kelp on lipid peroxidation and glutathione peroxidase activity in liv... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Volume: 238 ISSN: 0721-832X ISO Abbreviation: Graefes Arch. Clin. Exp. Ophthalmol. Publication Date: 2000 Jun |
Date Detail:
|
Created Date: 2000-11-08 Completed Date: 2000-11-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8205248 Medline TA: Graefes Arch Clin Exp Ophthalmol Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 496-9 Citation Subset: IM |
Affiliation:
|
Department of Anatomy, Christian Albrechts-University of Kiel, Germany. fpaulsen@anat.uni-kiel.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Absorption Animals Autoradiography Eye Proteins / metabolism Female Nasolacrimal Duct / cytology, metabolism* Ophthalmic Solutions Rats Rats, Wistar Serum Albumin, Bovine / administration & dosage, diagnostic use, metabolism* Tears / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Eye Proteins; 0/Ophthalmic Solutions; 0/Serum Albumin, Bovine; 0/tear proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increased growth factor activity in the aqueous humour of patients with exfoliation syndrome.
Next Document: Glutamine synthetase and marker enzymes of the blood-retina barrier in fetal bovine retinal pigment ...