Document Detail

What happens in freezing bodies? Experimental study of histological tissue change caused by freezing injuries.
MedLine Citation:
PMID:  10464930     Owner:  NLM     Status:  MEDLINE    
In order to evaluate histological features of freezing damages to human tissue after death, we froze samples of liver and heart tissue to temperatures of -12 degrees C, -28 degrees C and -80 degrees C, and stored them for 24 and 72 h, respectively, at those temperatures. After thawing and routine preparation for histology, the samples were evaluated both by microscope and with an electronic image analyzer. In all cases, we found extended extracellular spaces and shrunken cells resulting from the freeze-thaw cycle. These features were more pronounced in tissues stored for longer durations. Such findings seem to be typical of tissue that has been frozen prior to examination. Two cases of dead bodies found outdoors at subzero temperatures demonstrate that formerly frozen and unfrozen tissues can be distinguished histologically. The findings are examined in relation to the fundamental laws of cryobiology.
A T Schäfer; J D Kaufmann
Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  102     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  149-58     Citation Subset:  IM    
Institute of Forensic Medicine, RWTH Aachen, Germany.
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MeSH Terms
Hypothermia / pathology*
Liver / pathology*
Middle Aged
Myocardium / pathology*
Postmortem Changes*
Refrigeration / adverse effects*,  methods
Time Factors

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