Document Detail

What is the benefit to Escherichia coli of having multiple toxin-antitoxin systems in its genome?
MedLine Citation:
PMID:  17513477     Owner:  NLM     Status:  MEDLINE    
The Escherichia coli K-12 chromosome encodes at least five proteic toxin-antitoxin (TA) systems. The mazEF and relBE systems have been extensively characterized and were proposed to be general stress response modules. On one hand, mazEF was proposed to act as a programmed cell death system that is triggered by a variety of stresses. On the other hand, relBE and mazEF were proposed to serve as growth modulators that induce a dormancy state during amino acid starvation. These conflicting hypotheses led us to test a possible synergetic effect of the five characterized E. coli TA systems on stress response. We compared the behavior of a wild-type strain and its derivative devoid of the five TA systems under various stress conditions. We were unable to detect TA-dependent programmed cell death under any of these conditions, even under conditions previously reported to induce it. Thus, our results rule out the programmed-cell-death hypothesis. Moreover, the presence of the five TA systems advantaged neither recovery from the different stresses nor cell growth under nutrient-limited conditions in competition experiments. This casts a doubt on whether TA systems significantly influence bacterial fitness and competitiveness during non-steady-state growth conditions.
Virginie Tsilibaris; Geneviève Maenhaut-Michel; Natacha Mine; Laurence Van Melderen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-18
Journal Detail:
Title:  Journal of bacteriology     Volume:  189     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-21     Completed Date:  2007-10-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6101-8     Citation Subset:  IM    
Laboratoire de Génétique des Procaryotes, Institut de Biologie et Médecine Moléculaires, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, B-6041 Gosselies, Belgium.
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MeSH Terms
Acids / pharmacology
Adaptation, Physiological*
Amino Acids / metabolism
Anti-Bacterial Agents / pharmacology
Bacterial Toxins / genetics,  toxicity*
Colony Count, Microbial
DNA-Binding Proteins / genetics,  physiology
Endoribonucleases / genetics,  physiology
Escherichia coli / genetics,  growth & development*,  physiology
Escherichia coli Proteins / genetics,  physiology*
Gene Deletion
Microbial Viability*
Rifampin / pharmacology
Reg. No./Substance:
0/Acids; 0/Amino Acids; 0/Anti-Bacterial Agents; 0/Bacterial Toxins; 0/DNA-Binding Proteins; 0/DinJ protein, E coli; 0/Escherichia coli Proteins; 0/MazE protein, E coli; 0/MazF protein, E coli; 0/RelB protein, E coli; 0/RelE protein, E coli; 0/YafQ protein, E coli; 0/YefM protein, E coli; 0/YoeB protein, E coli; 13292-46-1/Rifampin; EC 3.1.-/Endoribonucleases; EC 3.1.27.-/ChpBK protein, E coli
Comment In:
J Bacteriol. 2007 Sep;189(17):6089-92   [PMID:  17616596 ]

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