Document Detail


What a study of pterygia teaches us about the cornea? Molecular mechanisms of formation.
MedLine Citation:
PMID:  20724855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments were carried out in the early 1990s to investigate the cell types involved in a pterygium and to determine a possible mechanism of formation. Our first experiments used monoclonal antibodies to keratins and an associated protein (vimentin), to look at the cells that compose a pterygium. These experiments demonstrated that a pterygium is the result of an abnormal limbal basal epithelial stem cell that moves onto Bowman's layer and brings about the dissolution of this layer. More importantly, these data showed that the clear corneal epithelial cells in front of the pterygium also contained these abnormal limbal cells, which we named the pterygium cell. This demonstrated that when a pterygium is removed, a wide area of what appears to be normal epithelium must be removed to inhibit reoccurrence of the growth. Later experiments using expressed sequence tag analysis of an un-normalized unamplified complementary DNA library from surgically removed pterygia were compared with normal cornea and confirmed the role of the epithelial cells in this growth. The gene expression studies also showed that genes involved in cellular migration are stimulated, and this led to studies on polyamine analogs as inhibitors of pterygial migration. Immunohistochemical studies with antibodies to matrix metalloproteinases (MMPs) showed that it is the pterygium cell that produces the MMPs that dissolve Bowman's layer resulting in the growth stimulation of stromal fibroblasts. This led to experiments on the use of MMP inhibitors to inhibit the growth of pterygia.
Authors:
Ted W Reid; Nicholas Dushku
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Eye & contact lens     Volume:  36     ISSN:  1542-233X     ISO Abbreviation:  Eye Contact Lens     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-08     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160941     Medline TA:  Eye Contact Lens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  290-5     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. ted.reid@ttuhsc.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cornea / metabolism*
Epithelium, Corneal
Fibroblasts / pathology
Humans
Matrix Metalloproteinases / antagonists & inhibitors,  metabolism
Protease Inhibitors / pharmacology
Pterygium / etiology*,  metabolism*,  prevention & control
Chemical
Reg. No./Substance:
0/Protease Inhibitors; EC 3.4.24.-/Matrix Metalloproteinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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