Document Detail


What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs.
MedLine Citation:
PMID:  23027043     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The design of small gene silencing RNAs with a high probability of being efficient still has some elements of an art, especially when the lowest concentration of small molecules needs to be utilized. The design of highly target-specific small interfering RNAs or short hairpin RNAs is even a greater challenging task. Some logical schemes and software tools that can be used for simplifying both tasks are presented here. In addition, sequence motifs and sequence composition biases of small interfering RNAs that have to be avoided because of specificity concerns are also detailed.
Authors:
Olga Matveeva
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  942     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Affiliation:
Department of Human Genetics, University of Utah, Salt Lake City, UT, USA, olga.matveeva@gmail.com.
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