| Western array analysis of cell cycle protein changes during the hyperplastic to hypertrophic transition in heart development. | |
| | |
MedLine Citation:
|
PMID: 17457520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cardiac myocytes proliferate most rapidly during the hyperplastic phase of heart development; however, the level of cell cycle activity is drastically down regulated after birth. Further growth of the heart is achieved by hypertrophic growth of cardiac myocytes. The mechanism that controls the switch from hyperplastic proliferation to hypertrophic growth in cardiac myocytes is unknown. Understanding this fundamental mechanism of cardiac myocyte biology would be most beneficial for studies directed towards myocardial regeneration. In this study, we identified changes in the expression of proteins involved in cell cycle regulation during the hyperplastic to hypertrophic transition of cardiac myocytes. Using a high-throughput immunoblotting technique, we examined 200+ proteins in primary cultures of cardiac myocytes at different developmental time points to determine the important regulators of this transition. In addition, we also analyzed samples from an immortalized cardiac myocyte cell line to compare expression levels of cell cycle regulatory proteins to our primary cultures. Our findings by this uncovered proteomic screen identified several potential key regulatory proteins and provide insight into the important components of cardiac myocyte cell cycle regulation. |
| | |
Authors:
|
Heather J Evans; Richard L Goodwin |
Publication Detail:
|
Type: Journal Article Date: 2007-04-25 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: 303 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-08-09 Completed Date: 2007-10-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 189-99 Citation Subset: IM |
Affiliation:
|
Department of Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC 29209, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Blotting, Western Cardiomegaly / metabolism* Cell Cycle / physiology* Cell Cycle Proteins / metabolism* Cells, Cultured Gene Expression Regulation, Developmental* Heart / growth & development Hyperplasia* Mice Myocardium / cytology, metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression and transcriptional activity of alternative splice variants of Mitf exon 6.
Next Document: Oxidative stress enhances phosphorylation of p53 in neonatal rat cardiomyocytes.