| Weightlessness induced apoptosis in normal thyroid cells and papillary thyroid carcinoma cells via extrinsic and intrinsic pathways. | |
| | |
MedLine Citation:
|
PMID: 12933692 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Apoptosis plays a pivotal role in development, tissue homeostasis, cancer, immune defense, and response to weightlessness. It can be initiated by external signals via death receptors, but may also emerge from mitochondria. We exposed mitochondria-rich thyroid carcinoma cells (ONCO-DG1 cell line) and normal thyroid cells (HTU-5) to conditions of simulated microgravity. After 24 h, 10% of the cancer cells had entered a Fas-dependent apoptotic pathway, but destruction and redistribution of mitochondria, microtubuli disruption, and caspase-3 activation were also detected, demonstrating the activation of extrinsic as well as intrinsic pathways. Furthermore, ONCO-DG1 cells grown on the clinostat showed elevated amounts of Bax, but reduced quantities of bcl-2. In addition, signs of apoptosis became detectable, as assessed by terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick end labeling, 4',6-diamidino-2-phenylindole staining, and 85-kDa apoptosis-related cleavage fragments. These fragments resulted from enhanced 116-kDa poly(ADP-ribose)polymerase activity and apoptosis. Apoptosis was also detected in normal HTU-5 cells, as demonstrated by electron microscopy, activation of caspase-3, increases in Fas and Bax, and elevation of 85-kDa apoptosis-related cleavage fragments resulting from enhanced poly(ADP-ribose) polymerase activity. Gravitational unloading affects the mitochondria and thereby may trigger apoptosis in thyroid cells subjected to weightlessness by clinorotation. |
| | |
Authors:
|
Peter Kossmehl; Mehdi Shakibaei; Augusto Cogoli; Manfred Infanger; Francesco Curcio; Johann Schönberger; Christoph Eilles; Johann Bauer; Holger Pickenhahn; Gundula Schulze-Tanzil; Martin Paul; Daniela Grimm |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Endocrinology Volume: 144 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2003 Sep |
Date Detail:
|
Created Date: 2003-08-22 Completed Date: 2003-09-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 4172-9 Citation Subset: AIM; IM; S |
Affiliation:
|
Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universität Berlin, 14195 Berlin, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, CD95
/
metabolism Apoptosis / physiology* Blotting, Western Carcinoma, Papillary* Caspase 3 Caspases / metabolism Collagen Type I / metabolism Extracellular Matrix Proteins / metabolism Fibronectins / metabolism Humans Laminin / metabolism Mitochondria / metabolism Osteopontin Poly(ADP-ribose) Polymerases / metabolism Rotation Sialoglycoproteins / metabolism Thyroid Gland / cytology* Thyroid Neoplasms* Tumor Cells, Cultured / cytology, metabolism Weightlessness* |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD95; 0/Collagen Type I; 0/Extracellular Matrix Proteins; 0/Fibronectins; 0/Laminin; 0/SPP1 protein, human; 0/Sialoglycoproteins; 106441-73-0/Osteopontin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Age-related changes in estrogen receptor beta in rat hypothalamus: a quantitative analysis.
Next Document: Expression of Bambi is widespread in juvenile and adult rat tissues and is regulated in male germ ce...