| Weight Loss via exercise with controlled dietary intake may affect phospholipid profile for cancer prevention in murine skin tissues. | |
| | |
MedLine Citation:
|
PMID: 20233900 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Exercise has been linked to a reduced cancer risk in animal models. However, the underlying mechanisms are unclear. This study assessed the effect of exercise with dietary consideration on the phospholipid profile in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin tissues. CD-1 mice were randomly assigned to one of the three groups: ad libitum-fed sedentary control; ad libitum-fed treadmill exercise at 13.4 m/min for 60 min/d, 5 d/wk (Ex+AL); and treadmill-exercised but pair-fed with the same amount as the control (Ex+PF). After 14 weeks, Ex+PF but not Ex+AL mice showed approximately 25% decrease in both body weight and body fat when compared with the controls. Of the total 338 phospholipids determined by electrospray ionization-tandem mass spectrometry, 57 were significantly changed, and 25 species could distinguish effects of exercise and diet treatments in a stepwise discriminant analysis. A 36% to 75% decrease of phosphatidylinositol (PI) levels in Ex+PF mice occurred along with a significant reduction of PI 3-kinase in TPA-induced skin epidermis, as measured by both Western blotting and immunohistochemistry. In addition, approximately 2-fold increase of the long-chain polyunsaturated fatty acids, docosahexaenoic and docosapentaenoic acids, in phosphatidylcholines, phosphatidylethanolamines, and lysophosphatidylethanolamines was observed in the Ex+PF group. Microarray analysis indicated that the expression of fatty acid elongase-1 increased. Taken together, these data indicate that exercise with controlled dietary intake, but not exercise alone, significantly reduced body weight and body fat as well as modified the phospholipid profile, which may contribute to cancer prevention by reducing TPA-induced PI 3-kinase and by enhancing omega-3 fatty acid elongation. |
| | |
Authors:
|
Ping Ouyang; Yu Jiang; Hieu M Doan; Linglin Xie; David Vasquez; Ruth Welti; Xiaoyu Su; Nanyan Lu; Betty Herndon; Shie-Shien Yang; Richard Jeannotte; Weiqun Wang |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-03-16 |
Journal Detail:
|
Title: Cancer prevention research (Philadelphia, Pa.) Volume: 3 ISSN: 1940-6215 ISO Abbreviation: Cancer Prev Res (Phila) Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-04-06 Completed Date: 2010-06-25 Revised Date: 2011-07-28 |
Medline Journal Info:
|
Nlm Unique ID: 101479409 Medline TA: Cancer Prev Res (Phila) Country: United States |
Other Details:
|
Languages: eng Pagination: 466-77 Citation Subset: IM |
Copyright Information:
|
(c) 2010 AACR. |
Affiliation:
|
Department of Human Nutrition, Kansas State University, Manhattan, KS 66506, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases
/
biosynthesis,
metabolism Animals Blotting, Western Body Weight Diet Eating / physiology* Female Gene Expression / physiology Immunohistochemistry Mice Oligonucleotide Array Sequence Analysis Phosphatidylinositol 3-Kinases / analysis, metabolism Phospholipids / analysis, metabolism* Physical Conditioning, Animal* Skin / chemistry Skin Neoplasms / metabolism*, prevention & control Weight Loss / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
P20 RR015563-050013/RR/NCRR NIH HHS; P20 RR016475/RR/NCRR NIH HHS; P20 RR15563/RR/NCRR NIH HHS; R01 CA106397/CA/NCI NIH HHS; R01 CA106397-01/CA/NCI NIH HHS; R01 CA106397-02/CA/NCI NIH HHS; R01 CA106397-03/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Phospholipids; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases; EC 2.7.1.-/Phosphatidylinositol 3-Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: STAT2 contributes to promotion of colorectal and skin carcinogenesis.
Next Document: 5-Deoxykaempferol Plays a Potential Therapeutic Role by Targeting Multiple Signaling Pathways in Ski...