| Wavelet analysis reveals heterogeneous time-dependent oscillations of individual mitochondria. | |
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MedLine Citation:
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PMID: 20833956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial inner membrane potential oscillations in cardiac myocytes synchronize under oxidative or metabolic stress, leading to synchronized whole cell oscillations. Gaining information about the temporal properties of individual mitochondrial oscillators is essential to comprehend the network's intrinsic spatiotemporal organization. We have developed methods to detect individual mitochondrial tetramethylrhodamine ethyl ester fluorescence oscillations and assess their dynamical properties using wavelet analysis. We demonstrate that these advanced signal processing tools can provide quantitative spatiotemporal information about intermitochondrial coupling. We have found that the mean frequency of selected groups of continuously oscillating mitochondria was 16.49 ± 1.04 mHz, whereas the mean frequency in the normalized mean global wavelet spectrum was 22.84 ± 1.80 mHz (n = 9 myocytes). In conclusion, this novel methodology will help shed new light on the dynamical properties of the mitochondrial network on the verge of synchronization. |
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Authors:
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Felix T Kurz; Miguel A Aon; Brian O'Rourke; Antonis A Armoundas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-10 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 299 ISSN: 1522-1539 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-01 Completed Date: 2010-11-29 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H1736-40 Citation Subset: IM |
Affiliation:
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Charité Universitätsmedizin Berlin, Campus Virchow-Klinikum, Klinik m.S. Kardiologie, Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Guinea Pigs Heart Ventricles / cytology Membrane Potential, Mitochondrial / physiology* Mitochondria, Heart / physiology* Myocytes, Cardiac / cytology Organometallic Compounds Signal Processing, Computer-Assisted* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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1R01-HL-103961/HL/NHLBI NIH HHS; 1R21-AG-035128/AG/NIA NIH HHS; R37 HL054598-16/HL/NHLBI NIH HHS; R37HL-54598/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Organometallic Compounds; 0/tetramethyl rhodamine ethyl ester |
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