Document Detail


Waveform modulation of negative-pressure wound therapy in the murine model.
MedLine Citation:
PMID:  21460654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Negative-pressure wound therapy applied with a porous foam interface has been shown to accelerate granulation-tissue formation when a cyclic application mode of suction is applied, but the optimal waveform has not been determined. The authors hypothesized that changes in the suction waveform applied to wounds would modulate the biological response of granulation tissue formation.
METHODS: A vacuum-assisted closure device (Kinetic Concepts, Inc., San Antonio, Texas) was applied to full-thickness wounds in 48 male diabetic mice (C57BL/KsJ-Lepr db), which were treated with six different waveforms: square waveforms of 125 mmHg of suction for 2 minutes, alternating with 50 mmHg of suction for 2 minutes, 5 minutes, or 10 minutes; triangular waveform with a 7-minute period oscillating between 50 and 125 mmHg; and static suction at 125 mmHg or static suction at 0 mmHg (occlusive dressing). Wounds were quantitatively evaluated for granulation tissue thickness as well as the number of proliferating cells and the number of blood vessels of the newly formed granulation tissue.
RESULTS: At 7 days, the continuous and triangular waveforms induced the thickest granulation tissue, with high rates of cellular proliferation and blood vessel counts compared with square wave and occlusive dressing control wounds. Decreasing square waveform frequency significantly increased granulation tissue thickness, cellular proliferation, and blood vessel counts.
CONCLUSIONS: Waveform modulation has a significant effect on granulation tissue formation, angiogenesis, and cellular proliferation in excisional wounds in diabetic mice. The rapid change in pressure seen in our square wave model may be detrimental to granulation tissue formation.
Authors:
Pouya Dastouri; Douglas L Helm; Saja S Scherer; Giorgio Pietramaggiori; George Younan; Dennis P Orgill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  127     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-04     Completed Date:  2011-05-26     Revised Date:  2014-10-13    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1460-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD31 / analysis
Cell Proliferation
Diabetes Mellitus, Experimental / physiopathology
Granulation Tissue / pathology,  physiology
Ki-67 Antigen / analysis
Male
Mice
Mice, Inbred C57BL
Negative-Pressure Wound Therapy / methods*
Neovascularization, Physiologic
Skin / blood supply,  injuries*
Suction / methods
Wound Healing / physiology*
Chemical
Reg. No./Substance:
0/Antigens, CD31; 0/Ki-67 Antigen
Comments/Corrections
Comment In:
Plast Reconstr Surg. 2011 Dec;128(6):784e-5e; author reply 785e-6e   [PMID:  22094790 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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