Document Detail

Water transport and cell survival in cryobiological procedures.
MedLine Citation:
PMID:  17873     Owner:  NLM     Status:  MEDLINE    
Living cells may be cooled to 77 K (liquid nitrogen) either to destroy them selectively or to store them for long periods. Water transport across the cell membranes during freezing and thawing is a primary factor determining whether the cells survive. These water movements are controlled by phase changes both intracellular and extracellular and by other factors such as the nature of any cryoprotective agent present, and the rates of cooling and thawing. The relation between cooling procedure, water transport and cell survival is discussed. In particular, the crucial rôle of dilution shock is emphasized: this is the damage to cells induced during the dilution that occurs both as ice melts during rewarming and when any cryoprotective additives are removed after thawing. Apart from the usefulness of understanding these processes for maximizing preservation or controlling selective destruction, the diverse responses of cells to different combinations of water transport and temperature changes appear likely to provide basic information on the properties of cell membranes.
J Farrant
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Philosophical transactions of the Royal Society of London. Series B, Biological sciences     Volume:  278     ISSN:  0962-8436     ISO Abbreviation:  Philos. Trans. R. Soc. Lond., B, Biol. Sci.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-08-12     Completed Date:  1977-08-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7503623     Medline TA:  Philos Trans R Soc Lond B Biol Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-205     Citation Subset:  IM    
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MeSH Terms
Biological Transport
Cell Line
Cell Survival / drug effects*
Cells / metabolism*
Cryoprotective Agents / pharmacology*
Freeze Etching
Osmolar Concentration
Time Factors
Water / metabolism*
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ice; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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