| Water-soluble compounds in the herbal preparation Abana inhibit lipid biosynthesis and enhance cholesterol efflux in HepG2 cells. | |
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MedLine Citation:
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PMID: 20555414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Higher concentrations of circulating lipids (cholesterol and triglycerides) and their decreased catabolism pose a major risk in the development of atherosclerosis and coronary heart disease (CHD). Although statins are widely used for treatment of hyperlipidemia, side effects associated with their use have prompted the search for a safer alternative for treating hyperlipidemia. The present study investigated the effect of water-soluble compounds in Abana (WSCA), a polyherbal drug formulation traditionally used in India for the treatment of hyperlipidemia, on lipid metabolism in HepG2 cells. WSCA reduced cholesterol and triglyceride content in the cells and their supernatant. WSCA inhibited the incorporation of [2-14C]acetate into cellular cholesterol and fatty acids, suggesting the inhibition of lipid synthesis. In addition, WSCA inhibited HMG-CoA reductase, a key metabolic enzyme involved in the biosynthesis of cholesterol. WSCA also increased cholesterol and fatty acid secretion into the cell supernatant, suggesting the enhanced removal of cholesterol and fatty acids. Furthermore, WSCA showed decreased linoleic acid (18:2) and arachidonic acid (20:4) content in HepG2 cells. The present study is the first to show that WSCA simultaneously inhibited cellular cholesterol biosynthesis and increased cholesterol secretion into the cell supernatant in HepG2 cells. |
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Authors:
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Satyakumar Vidyashankar; Ashok Godavarthi; R Sandeep Varma; Krishna S Nandakumar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 88 ISSN: 1205-7541 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-06-17 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: Canada |
Other Details:
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Languages: eng Pagination: 456-64 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Immunology, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 123, India. vidyashankar77@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Acyl Coenzyme A / metabolism Cell Survival / drug effects Cholesterol / analysis, biosynthesis, metabolism* Fatty Acids / metabolism Hep G2 Cells Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology* Lipids / biosynthesis*, pharmacology Medicine, Ayurvedic Microsomes / metabolism Plant Extracts / chemistry, pharmacology* Solubility Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Acyl Coenzyme A; 0/Fatty Acids; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Lipids; 0/Plant Extracts; 0/Triglycerides; 105863-96-5/abana; 1553-55-5/3-hydroxy-3-methylglutaryl-coenzyme A; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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