Document Detail


Water immiscible solvent based immunoassay.
MedLine Citation:
PMID:  9257263     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A competitive monoclonal antibody-based immunoassay which quantifies a hydrophobic hapten (Rx) in water immiscible solvents, obviating the need of a pre-extraction step, has been developed. Approximately linear dose response profiles of analyte, over the range 1-20 ugml-1 in the hydrophobic solvents, hexane, toluene and xylene were obtained. UV spectrophotometric analyses of Rx dosed hexane confirm the phenomenon of antibody-mediated transfer of analyte from the organic to the aqueous milieu. Preliminary data on the effect of water immiscible solvents on the immunoreactivity of a monoclonal antibody in free solution are presented. The potential industrial applications of water immiscible solvent based immunoassays are discussed.
Authors:
J P Aston; E J Hitchings; R L Ball; I Weeks; J S Woodhead
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunoassay     Volume:  18     ISSN:  0197-1522     ISO Abbreviation:  J Immunoassay     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-25     Completed Date:  1997-09-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8007167     Medline TA:  J Immunoassay     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-46     Citation Subset:  IM    
Affiliation:
Molecular Light Technology Research Ltd., Cardiff.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / chemistry
Dose-Response Relationship, Immunologic
Enzyme-Linked Immunosorbent Assay / methods*
Haptens / immunology
Humans
Serum Albumin / chemistry
Solubility
Solvents*
Ultraviolet Rays
Water*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Haptens; 0/Serum Albumin; 0/Solvents; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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