| Water dropwort (Ostericum sieboldii) and Sedum (Sedum sarmentosum) delay H(2)O(2)-induced senescence in human diploid fibroblasts. | |
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MedLine Citation:
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PMID: 19627195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that a close relationship exists among oxidative damage, senescence, and aging. Water dropwort (Ostericum sieboldii Miq. Nakai) and Sedum (Sedum sarmentosum Bunge) are popular green vegetables in Korea and are reported to have strong antioxidative activity. We investigated whether dropwort and Sedum have the potential to prevent aging using H(2)O(2)-induced prematurely senescent human diploid fibroblasts (HDFs). Dropwort and Sedum had similarly high contents of polyphenols, but dropwort had a flavonoid content about twofold higher than that of Sedum. Exposure of young HDFs to H(2)O(2) induced G2/M cell cycle arrest, positive senescence-associated (SA) beta-galactosidase (beta-gal) staining, and elevated p53, p21, and p16 protein levels. However, cotreatment with dropwort or Sedum ethanol extract significantly lowered p53, p21, and p16 levels and intracellular reactive oxygen species levels and attenuated the cell cycle arrest compared with H(2)O(2)-alone treatment. Interestingly, the increase in p16 level was prevented more quickly and clearly by dropwort treatment than Sedum treatment. The number of SA beta-gal-positive cells at 7 days after treatment was significantly reduced in dropwort-treated cells compared to H(2)O(2) alone-treated cells, whereas it was slightly reduced in Sedum-treated cells with no significance. In conclusion, dropwort showed a potential anti-senescence activity in H(2)O(2)-treated HDFs, which might be mediated by reducing p16, p21, and p53 levels and oxidative stress. |
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Authors:
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Sung Chae Moon; Sang Chul Park; Eui Ju Yeo; Chung Shil Kwak |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medicinal food Volume: 12 ISSN: 1557-7600 ISO Abbreviation: J Med Food Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-07-24 Completed Date: 2009-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812512 Medline TA: J Med Food Country: United States |
Other Details:
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Languages: eng Pagination: 485-92 Citation Subset: IM |
Affiliation:
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Institute on Aging, Seoul National University, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology* Apiaceae / chemistry* Cell Aging / drug effects* Cell Cycle / drug effects Cell Cycle Proteins / metabolism Cells, Cultured Diploidy Fibroblasts / drug effects* Flavonoids / pharmacology* Humans Hydrogen Peroxide / pharmacology Plant Extracts / pharmacology* Sedum / chemistry* beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Cell Cycle Proteins; 0/Flavonoids; 0/Plant Extracts; 7722-84-1/Hydrogen Peroxide; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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