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Water-Soluble Fluorescent Probes Based on endronized Polyfluorenes for Cell Imaging.
MedLine Citation:
PMID:  23364929     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Novel water-soluble dendronized fluorescent polyfluorenes (DFPFs) are prepared from hydrophilic monomers and hydrophobic comonomers. Incomplete energy transfer is found to result in a two-color emission of the DFPFs at around 410 and 650 nm. The incomplete energy transfer can be attributed to the poor compatibility between the fluorene and benzothiadiazole units. Polyethylene oxide (PEO) encapsulation of the DFPFs shows over 90% cell viability, indicating good biocompatibility. These DFPFs show differential cellular uptake. P1 with fewer PEO chains exhibits limited cellular membrane uptake and low brightness in cells. By contrast, P3 with more PEO chains is efficiently internalized by cells and accumulated in the cytoplasm. A strong fluorescence from whole cells is also observed.
Authors:
Libin Bai; Wei Li; Jingtian Chen; Fuli Bo; Baoxiang Gao; Hongmei Liu; Jingjing Li; Yonggang Wu; Xinwu Ba
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-31
Journal Detail:
Title:  Macromolecular rapid communications     Volume:  -     ISSN:  1521-3927     ISO Abbreviation:  Macromol Rapid Commun     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9888239     Medline TA:  Macromol Rapid Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
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