Document Detail


Water and CO(2) permeability of SsAqpZ, the cyanobacterium Synechococcus sp. PCC7942 aquaporin.
MedLine Citation:
PMID:  23289515     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Cyanobacteria possess Aquaporin-Z membrane channels (AqpZ) which have been suggested to mediate the water efflux underlying osmostress-inducible gene expression and to be essential for glucose metabolism under photomixotrophic growth. However, preliminary observations suggest that the biophysical properties of transport and physiological meaning of AqpZ in such photosynthetic microorganisms are not yet completely assessed. RESULTS: In this study, we used Xenopus laevis oocyte and proteoliposome systems to directly demonstrate the water permeability of the cyanobacterium Synechococcus sp. PCC7942 aquaporin, SsAqpZ. By an in-vitro assay of intracellular acidification in yeast cells SsAqpZ was found to transport also CO(2) . Consistent with this result, during the entire exponential phase of growth, Synechococcus SsAqpZ-null mutant cells grew slower than the corresponding wild type cells. This phenotype was stronger with higher levels of extracellular CO(2) . In line with the conversion of CO(2) gas into HCO(3) (-) ions under alkaline conditions, the impairment in growth of the SsAqpZ null strain was weaker in more alkaline culture medium. CONCLUSIONS: Cyanobacterial SsAqpZ may exert a pleiotropic function in addition to the already reported roles in macronutrient homeostasis and osmotic stress response as it appears to constitute an important pathway in CO(2) uptake, a fundamental step in photosynthesis.
Authors:
Xiaodong Ding; Tadashi Matsumoto; Patrizia Gena; Chengwei Liu; Marialuisa Pellegrini-Calace; Shihua Zhong; Xiaoli Sun; Yanming Zhu; Maki Katsuhara; Ikuko Iwasaki; Yoshichika Kitagawa; Giuseppe Calamita
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-6
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  -     ISSN:  1768-322X     ISO Abbreviation:  Biol. Cell     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Soçiété Francaise des Microscopies and Société de Biologie Cellulaire de France.
Affiliation:
Graduate School of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita, 010-0195, Japan; Plant Engineering Laboratory, College of Life Science, Northeast Agricultural University, Harbin, 150030, China; The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
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