Document Detail

Warthin-starry silver method showing particulate matter in macrophage.
MedLine Citation:
PMID:  18478983     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To verify whether Warthin-Starry (WS) silver method could detect the air particulate matter (PM)/dust particles (Ps) located within the macrophages in situ. METHODS: There were 26 autopsy cases that resulted from cerebral hemorrhage (group A), silicosis (group B), and fetal death during pregnancy (group C). Samples were collected separately and serial sections were prepared from the lungs and lymph nodes and stained with hematoxylin and eosin (HE), WS silver, immunohistochemistry of CD68. Furthermore, ultrathin sections were taken from the WS positive serial sections of groups A and B. Ps were observed under a transmission electron microscope (TEM) and the elements of Ps were measured by X-ray spectrum analysis (X-RSA). RESULTS: In both groups A and B, WS staining was positive for the larger and fine Ps, the so called "dust cells", but HE staining was almost negative for fine Ps. In group C, no larger or fine Ps were found. Immunohistochemical staining of CD68 certified that the "dust cells" containing Ps were macrophages. The results of TEM and X-RSA proved that the structure and elements of Ps belonged to PM indeed. CONCLUSION: WS staining is a better than HE staining in showing the location of PM within macrophages.
Hong-Gang Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  21     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-05-15     Completed Date:  2008-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-9     Citation Subset:  IM    
Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
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MeSH Terms
Macrophages / ultrastructure*
Microscopy, Electron, Transmission
Middle Aged
Silver Staining / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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