Document Detail


Warm water induces apoptosis, gonadal degeneration, and germ cell loss in subadult pejerrey Odontesthes bonariensis (Pisces, Atheriniformes).
MedLine Citation:
PMID:  18947325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Abstract Recent studies have shown that exposure to warm water can trigger gonadal degeneration and germ cell loss in fish of both sexes, but the mechanism behind this pathology is still not understood. This study was designed to characterize this process histologically and determine whether apoptosis plays any role during high temperature-induced gonadal cell degeneration in subadult pejerrey (Odontesthes bonariensis). For this purpose, fish were reared continuously at constant temperatures of 24 degrees C (control) and 29 degrees C (prolonged heat stress) or exposed for 36 h to 31 degrees C and then returned to 24 degrees C (short heat stress). Gonads were sampled at various times (hours, days, weeks) after the start of the experiment and were analyzed by light microscopy and stereometry for histological integrity/degeneration and germ cell counts, as well as by acridine orange fluorescence microscopy, TUNEL, and caspase activity assay for histochemical and biochemical signs of apoptosis. The results clearly implicate apoptosis in heat-induced somatic and germ cell degeneration in pejerrey and revealed that the dynamics and severity of this process were proportional to the magnitude of the thermal stress. Even a 36-h exposure to 31 degrees C induced significant increases in caspase-3 activity and number of apoptotic cells in both sexes, but males were shown to be more sensitive to heat stress than females.
Authors:
Lauro Satoru Ito; Chika Takahashi; Michiaki Yamashita; Carlos Augusto Strüssmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Physiological and biochemical zoology : PBZ     Volume:  81     ISSN:  1537-5293     ISO Abbreviation:  Physiol. Biochem. Zool.     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2008-11-03     Completed Date:  2009-01-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883369     Medline TA:  Physiol Biochem Zool     Country:  United States    
Other Details:
Languages:  eng     Pagination:  762-74     Citation Subset:  IM    
Affiliation:
Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Female
Fishes / physiology*
Germ Cells / cytology,  physiology
Hot Temperature / adverse effects*
Male
Ovary / cytology,  growth & development*
Sexual Maturation / physiology*
Testis / cytology,  growth & development*
Water
Chemical
Reg. No./Substance:
7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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