Document Detail


Wanted and unwanted properties of surface PEGylated nucleic acid nanoparticles in ocular gene transfer.
MedLine Citation:
PMID:  17574287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ocular gene therapy may offer new hope for severe eye diseases. Many of these ocular diseases are due to a gene defect in the retina, a multi-layered sensory tissue that lines the back of the eye. However, it is well known that the blood-retina barrier and sclera prevent hydrophilic and high molecular weight drugs to reach the retina after systemic or topical application. Therefore, intravitreal injection of non-viral nucleic acid nanoparticles has been considered as a safe and promising approach in ocular gene transfer. However, after intravitreal injection the non-viral nucleic acid nanoparticles should be stable and mobile in the vitreous. In this overview we focus on the behavior of non-viral nucleic acid nanoparticles (lipoplexes) in vitreous and on PEGylation strategies that improve their behavior in vitreous, but that do not affect their transfection capacity.
Authors:
Niek N Sanders; Liesbeth Peeters; Ine Lentacker; Joseph Demeester; Stefaan C De Smedt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2007-05-13
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  122     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-21     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  226-35     Citation Subset:  IM    
Affiliation:
Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. Niek.Sanders@UGent.be
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Drug Carriers* / administration & dosage,  adverse effects,  chemistry
Drug Stability
Epithelial Cells / drug effects,  metabolism
Eye Diseases / therapy
Gene Therapy / methods
Gene Transfer Techniques*
Humans
Liposomes
Luciferases, Firefly / genetics
Microbubbles
Nanoparticles* / administration & dosage,  adverse effects,  chemistry
Nucleic Acids / administration & dosage*,  genetics
Pigment Epithelium of Eye / cytology
Polyethylene Glycols* / administration & dosage,  adverse effects,  chemistry
Transfection
Ultrasonics
Vitreous Body / drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Liposomes; 0/Nucleic Acids; 0/Polyethylene Glycols; EC 1.13.12.7/Luciferases, Firefly

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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