Document Detail


Walker 256 cancer cells secrete tissue inhibitor of metalloproteinase-free metalloproteinase-9.
MedLine Citation:
PMID:  19330523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Walker 256 (W256) cancer cells, developed as ascites in rats, in response to endogenous unidentified stimuli, secrete a gelatinase of apparent molecular mass of 94 kDa, immunologically homologous to the zymogen of matrix metalloproteinase-9 (proMMP-9). After treatment with the activating agent 4-aminophenylmercuric acetate (APMA), affinity-purified W256 gelatinase is converted to a final processed form of 66 kDa in a similar fashion to TIMP-free human proMMP-9. It is demonstrated that although being capable of binding TIMP-1, W256 proMMP-9 is secreted from W256 cells in TIMP-free forms (monomers or oligomers). Moreover, using biochemical and immunological methods, it is established that the W256 cells do not express or secrete TIMP-1 protein, although RT-PCR analysis indicated low-level TIMP-1 mRNA expression. W256 cancer cells displayed high metastatic ability in rats that may be attributed in part to secretion of TIMP-free proMMP-9.
Authors:
Maria Pavlaki; Eleftheria Giannopoulou; Anna Niarakis; Panagiota Ravazoula; Alexios J Aletras
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Publication Detail:
Type:  Journal Article     Date:  2009-03-29
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  328     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-01     Completed Date:  2009-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  189-99     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Patras, Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma 256, Walker / secretion*
Cell Line, Tumor
Gelatinases
Humans
Matrix Metalloproteinase 9 / secretion*
Neoplasm Metastasis
Phenylmercuric Acetate / analogs & derivatives,  pharmacology
RNA, Messenger / analysis
Rats
Tissue Inhibitor of Metalloproteinase-1 / genetics,  secretion*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 62-38-4/Phenylmercuric Acetate; 6283-24-5/4-aminophenylmercuriacetate; EC 3.4.24.-/Gelatinases; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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